Screening of reference genes in tiger puffer (Takifugu rubripes) across tissues and under different nutritional conditions
文献类型： 外文期刊
作者： Liao, Zhangbin 1 ; Sun, Zhiyuan 2 ; Bi, Qingzhu 2 ; Gong, Qingli 1 ; Sun, Bo 2 ; Wei, Yuliang 2 ; Liang, Mengqing 2 ; Xu, 1 ;
作者机构： 1.Ocean Univ China, Fisheries Coll, 5 Yushan Rd, Qingdao 266003, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 106 Nanjing Rd, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, 1 Wenhai Rd, Qingdao 266237, Peoples R China
关键词： Reference gene; Takifugu rubripes; qRT-PCR; Starvation; Lipid levels
期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）
ISSN： 0920-1742
年卷期： 2021 年 47 卷 6 期
页码：
收录情况： SCI
摘要： The present study was aimed at screening suitable reference genes for quantitative real-time polymerase chain reaction (qRT-PCR) in tiger puffer (Takifugu rubripes), an important aquaculture species in Asia and also a good model species for lipid research. Specifically, this reference gene screening was targeted at standardization of gene expression in different tissues (liver, muscle, brain, intestine, heart, eye, skin, and spleen) or under different nutritional conditions (starvation and different dietary lipid levels). Eight candidate reference genes (ribosomal protein L19 and L13 (RPL19 and RPL13), elongation factor-1 alpha (EF1 alpha), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), hypoxanthine guanine phosphoribosyl transferase1 (HPRT1), beta-2-Microglobulin (B2M), 18S ribosomal RNA (18SrRNA), and beta actin (ACTB)) were evaluated with four algorithms (geNorm, NormFinder, BestKeeper, and comparative Delta Ct method). The results showed that different algorithms generated inconsistent results. Based on these findings, RPL19, EF1 alpha, 18SrRNA, and RPL13 were relatively stable in different tissues of tiger puffer. During starvation conditions, ACTB/RPL19 was the best reference gene combination. Under different dietary lipid levels, ACTB/RPL13 was the most suitable reference gene combination. The present results will help researchers to obtain more accurate results in future qRT-PCR analysis in tiger puffer.
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