FK506 Targets MoFpr1 to Modulate Autophagy and Ubiquitination, Inhibiting the Pathogenicity of Magnaporthe oryzae
文献类型： 外文期刊
作者： Zhao, Wen-Hui 1 ; Yan, Jiong-Yi 1 ; Xie, Ke-Xin 1 ; Wu, Xi-Yu 3 ; Qian, Hui 4 ; Luo, Xi-Chun 1 ; Liao, Jian 1 ; An, Chun-Yue 1 ; Liang, Shuang 2 ; Lu, Jian-Ping 5 ; Khan, Irshad Ali 6 ; Zhu, Xue-Ming 2 ; Lin, Fu-Cheng 2 ; Liu, Xiao-Hong 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Qual & Safety Agroprod, Zhejiang Prov Key Lab Agr Microbi, Hangzhou 310058, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Qual & Safety Agro Prod, Zhejiang Prov Key Lab Agr Microbi,Key Lab Agr Micr, Hangzhou 310021, Peoples R China
3.Xianghu Lab, Hangzhou 311231, Peoples R China
4.Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Peoples R China
5.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Peoples R China
6.Univ Swabi, Dept Agr Plant Pathol, Anbar 23561, Khyber Pakhtunk, Pakistan
关键词： FK506; X-ray crystallography; riceblast; broad-spectrum antifungal activity
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年 73 卷 24 期
页码：
收录情况： SCI
摘要： Rice blast, caused by Magnaporthe oryzae, is a major threat to global rice production. This study explores the antifungal potential of the immunosuppressant FK506 and identifies its target protein, MoFpr1 (FK506-binding protein 1B). FK506 inhibited mycelial growth, appressorium formation, and pathogenicity of M. oryzae in an MoFpr1-dependent manner. Mechanistic analyses revealed that FK506 impairs autophagy and ubiquitination, supported by transcriptomic and metabolomic data. Structural studies using X-ray crystallography and site-directed mutagenesis confirmed the direct interaction between FK506 and MoFpr1, highlighting the importance of residues Gly95 and Ile97. Furthermore, FK506 demonstrated broad-spectrum antifungal activity against various plant pathogens and effectively controlled rice blast in laboratory, net-chamber, and field trials with minimal phytotoxicity. These findings position FK506 as a promising antifungal agent and offer insights into its molecular mechanism, suggesting its potential for sustainable plant disease management.
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