A universal probe for simultaneous detection of six pospiviroids and natural infection of potato spindle tuber viroid (PSTVd) in tomato in China
文献类型： 外文期刊
作者： Yu-hong, Zhang 1 ; Zhi-xin, Li 2 ; Ya-jie, Du 1 ; Shi-fang, Li 1 ; Zhi-xiang, Zhang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Heilongjiang Acad Agr Sci, Keshan Branch, Qiqihar 161005, Peoples R China
3.Hebei Normal Univ Sci & Technol, Coll Agron & Biotechnol, Qinhuangdao 066004, Peoples R China
关键词： tomato; viroid; simultaneous detection; molecular hybridization; pospiviroids
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.8； 五年影响因子：4.8 ）
ISSN： 2095-3119
年卷期： 2023 年 22 卷 3 期
页码：
收录情况： SCI
摘要： Several viroids in the genus Pospiviroid can infect tomato (Solanum lycopersicum) and cause severe diseases, posing a serious threat to tomato production. For simultaneous detection of six tomato-infecting pospiviroids -columnea latent viroid (CLVd), pepper chat fruit viroid (PCFVd), potato spindle tuber viroid (PSTVd), tomato apical stunt viroid (TASVd), tomato chlorotic dwarf viroid (TCDVd), and tomato planta macho viroid (TPMVd), we developed a universal probe based on a highly conserved 61 nt long sequence shared among them. Compared with their specific probes, the universal probe has a similar, though slightly reduced, detection sensitivity and has the advantages of simple and cost-effective preparation and simultaneous detection of the six pospiviroids. In addition, the universal probe was used in dot-blot hybridization assays for a large-scale survey of viroid(s) in tomato plantings in China. Only PSTVd was detected in a few greenhouse-planted tomato plants. Sequence analysis revealed that these tomato PSTVd isolates may have been introduced from tomato seeds imported from abroad.
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