Unlocking the genetic basis of vitamin E content in sweet corn kernels: Expanding breeding targets through genome-wide association studies
文献类型： 外文期刊
作者： Li, Kun 1 ; Yu, Yongtao 1 ; Zhang, Nan 1 ; Xie, Lihua 1 ; Huang, Wenjie 4 ; Qi, Xitao 1 ; Li, Wu 1 ; Li, Chunyan 1 ; Wen, Tianxiang 1 ; Zhu, Wenguang 1 ; Yan, Shijuan 4 ; Li, Gaoke 1 ; Guo, Xinbo 3 ; Hu, Jianguang 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Crop Res Inst, Guangzhou, Peoples R China
2.Guangdong Key Lab Crop Genet Improvement, Guangzhou, Peoples R China
3.South China Univ Technol, Engn Res Ctr Starch & Vegetable Prot Proc, Sch Food Sci & Engn, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Guangzhou, Peoples R China
4.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou 510640, Peoples R China
关键词： Sweet corn; GWAS; Tocopherols; Tocotrienols; CRISPR-Cas9
期刊名称：PLANT SCIENCE （ 影响因子：4.1； 五年影响因子：5.1 ）
ISSN： 0168-9452
年卷期： 2024 年 348 卷
页码：
收录情况： SCI
摘要： Tocochromanols, collectively known as Vitamin E, serve as natural lipid-soluble antioxidants that are exclusively obtained through dietary intake in humans. Synthesized by all plants, tocochromanols play an important role in protecting polyunsaturated fatty acids in plant seeds from lipid peroxidation. While the genes involved in tocochromanol biosynthesis have been fully elucidated in Arabidopsis thaliana, Oryza sativa and Zea mays, the genetic basis of tocochromanol accumulation in sweet corn remains poorly understood. This gap is a consequence of limited natural genetic diversity and harvest at immature growth stages. In this study, we conducted comprehensive genome-wide association studies (GWAS) on a sweet corn panel of 295 individuals with a highdensity molecular marker set. In total, thirteen quantitative trait loci (QTLs) for individual and derived tocochromanol traits were identified. Our analysis identified novel roles for three genes, ZmCS2, Zmshki1 and ZmB4FMV1, in the regulation of alpha-tocopherol accumulation in sweet corn kernels. We genetically validated the role of Zmshki1 through the generation of a knock-out line using CRISPR-Cas9 technology. Further gene-based GWAS revealed the function of the canonical tyrosine metabolic enzymes ZmCS2 and Zmhppd1 in the regulation of total tocochromanol content. This comprehensive assessment of the genetic basis for variation in vitamin E content establishes a solid foundation for enhancing vitamin E content not only in sweet corn, but also in other cereal crops.
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