您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Iron-metabolic function and potential antibacterial role of Hepcidin and its correlated genes (Ferroportin 1 and Transferrin Receptor) in Turbot (Scophthalmus maximus)

文献类型： 外文期刊

作者： Yang, Chang-Geng 1 ; Liu, Shan-Shan 1 ; Sun, Bing 1 ; Wang, Xian-Li 4 ; Wang, Na 1 ; Chen, Song-Lin 1 ;

作者机构： 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

3.Dalian Fisheries Univ, Minist Agr, Key Lab Mariculture & Biotechnol, Dalian 116023, Peoples R China

4.Tongji Univ, Sch Med, Stem Cell Res Ctr, Translat Ctr Stem Cell Res,Tongji Hosp, Shanghai 200065, Peoples R China

关键词： Hepcidin;Iron-metabolism;Turbot;NF-kappa B;Immunity

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2013 年 34 卷 3 期

页码：

收录情况： SCI

摘要： Antimicrobial peptide plays an important role in fish immunity. The small molecular antimicrobial peptide Hepcidin in turbot was studied and reported in this paper. The Ferroportin 1 (FPN1) and Transferrin Receptor (TFR) genes, which are related to Hepcidin, were cloned in turbot. The characteristics of Hepcidin and its related genes were studied, including an analysis of the expression patterns and cloning of the Hepcidin promoter, the relationship between Hepcidin and NF-kappa B and the regulation of iron-metabolism. The results showed that the promoter of SmHepcidin contains the binding sites of NF-kappa B, and NF-kappa B may directly or indirectly receive feedback signals from SmHepcidin. In the liver, spleen and kidney, in which there was an increased SmHepcidin expression level, SmFPN1 dramatically decreased and SmTFR was also either decreased or exhibited no obvious change after bacterial/viral infection and an injection of exogenous Hepcidin protein. RNAi experiments in turbot kidney cells confirmed the expression changes of these gene patterns. Furthermore, the administration of exogenous Hepcidin protein, which regulates the level of chelatable iron in cells, further confirmed the function of Hepcidin in iron metabolism. It is speculated that the rapidly increased expression of SmHepcidin may induce changes in the expression of related genes, and that the in vivo chelatable iron concentration which participates in the antibacterial process was also changed when exogenous pathogens are present in turbot. It is suggested that SmHepcidin plays a defensive role against pathogenic infection. (C) 2012 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Molecular cloning and expression analysis of a hepcidin antimicrobial peptide gene from turbot (Scophthalmus maximus). Chen, Song-Lin,Li, Wei,Meng, Liang,Sha, Zhen-Xia,Wang, Zhi-Jian,Ren, Guo-Cheng. 2007

[2]Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus). Yang, Chang-Geng,Sun, Bing,Liu, Shan-Shan,Chen, Song-Lin,Yang, Chang-Geng,Wang, Xian-Li,Zhang, Bo. 2013

[3]大菱鲆抗菌肽基因酵母表达载体的构建及表达. 李伟,陈松林. 2008

[4]大菱鲆HEPCIDIN抗菌肽基因的克隆及在大肠杆菌中的高效表达. 李伟,陈松林,赵晓杰. 2007

[5]牙鲆抗菌肽HEPCIDIN基因的克隆及表达分析. 李伟,陈松林,张玉喜. 2007

[6]大菱鲆抗菌肽基因酵母表达载体的构建及表达. 李伟,陈松林. 2008

[7]牙鲆抗菌肽HEPCIDIN基因在大肠杆菌中的融合表达. 李伟,陈松林. 2007

[8]锦鲤Hepcidin基因的克隆及其组织特异性表达分析（英文）. 马志宏,姜娜,邢薇,李铁梁,袁丁,李文通,李炯棠,罗琳. 2017

[9]大口黑鲈hepcidin真核表达载体构建及表达的初步研究. 李胜杰,白俊杰,刘碧莲,叶星,于凌云,全迎春. 2009

[10]大口黑鲈抗菌肽hepcidin cDNA序列和结构分析. 白俊杰,刘碧莲,劳海华,叶星,简清. 2008

[11]鳜hepcidin cDNA的分子克隆及序列分析. 刘碧莲,白俊杰,劳海华,叶星,简清. 2006

[12]大菱鲆hepcidin抗菌肽基因的克隆及在大肠杆菌中的高效表达. 李伟,陈松林,赵晓杰. 2007

[13]Molecular identification and recombinant expression of red sea bream (Chrysophrys major) hepcidin gene. Chen, Song-Lin,Sui, Shao-Fei,Xu, Mei-Yu,Liu, Yang,Yang, Rong. 2005

[14]Molecular cloning and mRNA expression of a hepcidin gene from the spinyhead croaker, Collichthys lucidus. Sang, C.,Lin, Y.,Jiang, K.,Zhang, F.,Song, W.. 2015

[15]Characterization of the Mx and hepcidin genes in Epinephelus akaara asymptomatic carriers of the nervous necrosis virus. Mao, Ming-Guang,Jiang, Jie-Lan,Wang, Ke-Jian,Lei, Ji-Lin,Lei, Ji-Lin,Mao, Ming-Guang,Lei, Ji-Lin,Peralvarez-Marin, Alex. 2013

[16]Molecular characterization and expression analysis of the I kappa B gene from pearl oyster Pinctada fucata. Xu, Xinping,Zhang, Dianchang,Jiang, Shigui,Qiu, Lihua,Su, Tianfeng,Wu, Kaichang,Li, Youning,Zhu, Caiyan,Zhang, Dianchang. 2009

[17]Mycoplasma hyopneumoniae induces pro-inflammatory cytokine and nitric oxide production through NF kappa B and MAPK pathways in RAW264.7 cells. Hwang, Mi-Hyun,Damte, Dereje,Lee, Joong-Su,Gebru, Elias,Chang, Zhi-Qiang,Rhee, Man-Hee,Park, Seung-Chun,Cheng, Henrique,Jung, Byeong-Yeal,Chang, Zhi-Qiang. 2011

[18]Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus. Zhang, Yu-Xi,Zhang, Bo,Chen, Song-Lin,Wang, Xian-Li,Yang, Chang-Geng. 2012

[19]Isolation and analysis of microsatellites in the genome of turbot (Scophthalmus maximus L.). Ruan, Xiaohong,Hu, Jingjie,Ruan, Xiaohong,Wang, Weiji,Kong, Jie. 2011

[20]Cloning, characterization, and expression of a novel member of proteasomal subunits gene in turbot, Scophthalmus maximus. Zhang Bo,Song Wenping,Zheng Debin,Ma Chao,Xiao Guangxia,Wang Xianli. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台