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Picomol Assay of Nitric Oxide Screening in Biological Samples by Derivatization Combined with High-throughput Microplate Format

文献类型： 外文期刊

作者： Zhang, Xiaoling 1 ; Yang, Qiao 1 ;

作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab East China Sea & Ocean Fishery Resources, Sci Observing & Expt Stn Fisheries Resources & En, Shanghai 200090, Peoples R China

关键词： Nitric oxide;Biological samples;High-throughput;Microplate format;Derivatization

期刊名称：APPLIED MATERIALS AND TECHNOLOGIES FOR MODERN MANUFACTURING, PTS 1-4

ISSN： 1660-9336

年卷期： 2013 年 423-426 卷

页码：

收录情况： SCI

摘要： Nitric oxide (NO) is a critical intra- and extra-cellular signaling molecule that mediates a broad variety of physiologic and pathophysiologic events, and has prompted rapid growth of investigations in investigating its physiology and generation mechanism of corresponding diseases. In this paper, a picomol assay of NO screening method using fluorescent derivatization combined with high-throughout microplate format has been developed and successfully applied to the determination of NO in biological samples. The satisfied detection limit and recovery of the proposed method demonstrates that it can be competent for the sensitive determination of NO in extremely small volume of biological sample.

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[1]Determination of N-epsilon-Carboxymethyl-lysine Content in Muscle Tissues of Turbot by Gas Chromatography-Mass Spectrometry. Wang Jing,Tian Sheng-Lan,Lin Hong,Li Zhen-Xing,Sun Wei-Hong. 2015

[2]Selective and sensitive determination of pheomelanin in biological samples using MEKC with laser-induced fluorescence detection based on intramolecular excimer-forming fluorescence derivatization. Zhang, Xiaoling,Yang, Qiao,Yuan, Bin.

[3]Establishment of a Sensitive Nitric Oxide Bioassay for Bioscreening Application. Yang, Qiao,Zhang, Xiaoling,Huang, Hongliang. 2014

[4]Development of the Reliable Fluorometric Assay for the Sensitive Determination of Nitric Oxide Metabolites in Biological Fluids. Yang, Qiao,Zhang, Xiaoling,Huang, Hongliang. 2014

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