Simultaneous Determination of the residues of 25 Sex hormones in Aquatic Products by HPLC-ESI MS/MS
文献类型： 外文期刊
作者： Yu, Hui-juan 1 ; Zhang, Shan-shan 1 ; Feng, Bing 1 ; Huang, Dong-mei 1 ; Li, Bing 2 ;
作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
2.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai, Peoples R China
关键词： High performance liquid chromatography-tandem mass spectrometric;Androgen;Progestogen;Estrogen
期刊名称：ADVANCES IN CHEMICAL ENGINEERING III, PTS 1-4
ISSN： 1022-6680
年卷期： 2013 年 781-784 卷
页码：
收录情况： SCI
摘要： A high performance liquid chromatography-tandem mass spectrometric method had been established for simultaneous determination of 25 sex hormones. The hormones were extracted by ethyl acetate and ethyl acetate-tert-butyl methyl ether (1/1, v/v) after enzymatic hydrolysis, followed by an LC-C18 cartridge for cleanup, then were quantified by HPLC-ESI MS/MS with internal standards. Limits of quantification were 1-2 mu g.kg(-1), average recoveries were 92-113 %, and the R.S.D. was 6-10 %.
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