中国水产科学研究院机构知识库

Isolation and characterization of polymorphic microsatellite markers from Coilia ectenes

收藏
分享
全文链接

文献类型：外文期刊

作者： Rong, X. J. ¹ ; Xu, Y. J. ² ; Wang, Q. Y. ¹ ; Liao, M. J. ² ; Liu, X. Z. ² ; Pan, C. Y. ² ; Zhang, Z. ² ; Wang, Y. G.;

作者机构： 1.Ocean Univ China, Qingdao, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao, Peoples R China

关键词： Coilia ectenes;Microsatellite marker;Genetic diversity

期刊名称：GENETICS AND MOLECULAR RESEARCH （影响因子：0.764；五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2013 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Coilia ectenes (Jordan and Seale 1905) is an important anadromous species that is an important resource at risk of extinction because of over-fishing, pollution, and coastal construction. To evaluate the genetic diversity of C. ectenes for use in breeding programs, elite microsatellite-enriched libraries were constructed and novel microsatellite markers were developed, and applied to genetically detect wild populations. Out of 92 randomly selected and sequenced clones, 89 contained a CA or GA repeat motif. Twenty-two pairs of primers were designed to investigate the polymorphism and genetic structure of a wild population collected from the Yellow River estuary, China. It was found that 2 loci were monomorphic and 20 loci were polymorphic. The number of alleles per polymorphic loci ranged from 3 to 13, with an average of 7.9. The expected heterozygosity per locus ranged from 0.05 to 0.89, with an average of 0.68. The isolated polymorphic markers are expected to be of use in future genetic breeding programs for C. ectenes, and in the assessment of genetic variation within this species.

相关文献

[1]Genetic variation of Coilia ectenes (Clupeiformes: Engraulidae) revealed by the complete cytochrome b sequences of mitochondrial DNA. Ma, Chunyan,Cheng, Qiqun,Zhang, Qingyi,Zhuang, Ping,Ma, Chunyan,Zhao, Yunlong,Zhang, Qingyi.

[2]Genetic diversity and demographical history of Coilia ectenes (Clupeiformes: Engraulidae) inferred from the complete control region sequences of mitochondrial DNA. Ma, Chunyan,Cheng, Qiqun,Zhang, Qingyi.

[3]PCR-RFLP analysis of cytochrome b gene does not support Coilia ectenes taihuensis being a subspecies of Coilia ectenes. Cheng, QQ,Lu, DR.

[4]Genetic diversity analysis of oriental river prawn, Macrobrachium nipponense, in Yellow River using microsatellite marker. Qiao, H.,Jiang, S. F.,Sun, M.,Gong, Y. S.,Xiong, Y. W.,Jin, S. B.,Fu, H. T.,Lv, D.. 2013

[5]Development of microsatellite markers and their utilization in genetic diversity analysis of cultivated and wild populations of the mud carp (Cirrhina molitorella). Yang, Cheng,Sun, Xiaowen,Yang, Cheng,Zhu, Xinping. 2008

[6]Reconstructing habitat use of Coilia mystus and Coilia ectenes of the Yangtze River estuary, and of Coilia ectenes of Taihu Lake, based on otolith strontium and calcium. Yang, J.,Arai, T.,Liu, H.,Miyazaki, N.,Tsukamoto, K.. 2006

[7]Development of thirty-four novel polymorphic microsatellite markers in Coilia ectenes (Clupeiformes: Engraulidae) and cross-species amplification in two closely related taxa. Chen, Fang,Zeng, Liyan,Cheng, Qiqun,Chen, Fang. 2012

[8]Morphological differences between close populations discernible by multivariate analysis: A case study of genus Coilia (Teleostei : Clupeiforms). Cheng, QQ,Lu, DR,Ma, L. 2005

[9]Genetic structure and differentiation of four lake populations of Coilia ectenes (Clupeiformes: Engraulidae) based on mtDNA control region sequences. Cheng, Qiqun,Zhang, Qingyi,Ma, Chunyan,Guan, Weibing.

[10]The complete mitochondrial genome sequence of Coilia ectenes (Clupeiformes: Engraulidae). Qiao, Huiying,Cheng, Qiqun,Chen, Ying,Chen, Wenming,Zhu, Yuxia,Qiao, Huiying,Chen, Ying,Chen, Wenming,Zhu, Yuxia.

[11]In-depth transcriptome analysis of Coilia ectenes, an important fish resource in the Yangtze River: de novo assembly, gene annotation. Shen, Huaishun,Gu, Ruobo,Xu, Gangchun,Xu, Pao,Nie, Zijuan,Shen, Huaishun,Xu, Pao,Hu, Yacheng.

[12]Genomic structure, polymorphism and expression analysis of the growth hormone (GH) gene in female and male Half-smooth tongue sole (Cynoglossus semilaevis). Ma, Qian,Liu, ShuFang,Zhuang, ZhiMeng,Lin, Lin,Sun, ZhongZhi,Liu, ChangLin,Ma, Hui,Tang, QiSheng,Ma, Qian,Lin, Lin,Su, YongQuan,Ma, Hui.

[13]Identification of twenty novel polymorphic microsatellite DNA markers from transcripts of the pearl oyster Pinctada fucata using next-generation sequencing approach. Fan, Sigang,Wang, Jingxuan,Huang, Guiju,Liu, Baosuo,Yu, Dahui,Wang, Jingxuan. 2015

[14]Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin. Liu, Y. -X.,Ma, C. -Y.,Ma, H. -Y.,Feng, C. -L.,Li, S. -J.,Ma, L. -B.,Liu, Y. -X.. 2015

[15]Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers. Chang Yumei,Ding Lei,Liang Liqun,Chang Yumei,He Jianguo,Ding Lei,Li Mingyun,Xue Liangyi,Liang Liqun,Lei Qingquan. 2008

[16]Parentage determination of Chinese shrimp (Fenneropenaeus chinensis) based on microsatellite DNA markers. Dong, Shirui,Kong, Jie,Zhang, Tianshi,Meng, Xianhong,Wang, Rucai. 2006

[17]Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus. Lin, L.,Li, C. H.,Xu, S. N.,Liu, Y.,Xiao, Y. Y.,Lin, L.,Li, C. H.,Xu, S. N.,Liu, Y.,Xiao, Y. Y.. 2015

[18]Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers. Liu Shuaishuai,Ye Naihao,Liu Yunguo,Liu Yunguo,Liu Shuaishuai,Guo Yinghui,Li Junfeng. 2011

[19]Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes. Xu, Y. -J.,Liao, M. -J.,Wang, Y. -G.,Liu, Z. -C.,Qin, P.,Zhang, Z.,Rong, X. -J.,Liu, X. -Z.. 2013

[20]Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho. Liu, Shufang,Liu, Hongbo,Yuan, Yanjiao,Dai, Fangqun,Zhuang, Zhimeng,Liu, Hongbo,Lin, Lin,Yuan, Yanjiao. 2012

作者其他论文更多>>

Effects of dietary supplementation of A3 alpha peptidoglycan on the growth, immune response and defence of sea cucumber

作者：Zhang, C. Y.;Chen, G. F.;Wang, C. C.;Xu, Z.;Song, X. L.;Wang, Y. G.

关键词：A3 alpha-peptidoglycan;Apostichopus japonicus;dietary supplementation;growth;immune response;Vibrio splendidus
Genetic diversity of different populations and improved growth in the F1 hybrids in the swimming crab (Portunus trituberculatus)

作者：Gao, B. Q.;Liu, P.;Li, J.;Wang, Q. Y.;Li, X. P.;Gao, B. Q.

关键词：Portunus trituberculatus;Geographic population;Heterosis;Genetic differentiation;Crossbreeding
Edwardsiellosis in farmed turbot, Scophthalmus maximus (L.), associated with an unusual variant of Edwardsiella tarda: a clinical, aetiological and histopathological study

作者：Qin, L.;Xu, J.;Wang, Y. G.

关键词：granuloma;turbot Scophthalmus maximus (L.);macrophage;histopathology;non-flagellated Edwardsiella tarda
Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes

作者：Xu, Y. -J.;Liao, M. -J.;Wang, Y. -G.;Liu, Z. -C.;Qin, P.;Zhang, Z.;Rong, X. -J.;Liu, X. -Z.

关键词：Paralichthys lethostigma;Microsatellite marker;Cross-species amplification;Pleuronectiformes
CLONING AND CHARACTERIZATION OF A TRANSCRIPTIONAL REPRESSOR FROM FENNEROPENAEUS CHINENSIS (OSBECK, 1765)

作者：Lai, X. F.;Gao, H.;Lai, X. F.;Gao, H.;Kong, J.;Wang, Q. Y.;Wang, W. J.;Meng, X. H.

关键词：
A3 alpha-peptidoglycan extracted from Bifidobacterium sp could enhance immunological ability of Apostichopus japonicus

作者：Zhang, C. Y.;Chen, G. F.;Wang, Y. Y.;Xu, Z.;Wang, Y. G.;Song, X. L.

关键词：A3 alpha-peptidoglycan;Apostichopus japonicus;hypodermic injection;immunological ability;Vibrio splendidus
IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF A CHITIN BINDING-LIKE PROTEIN FROM FENNEROPENAEUS CHINENSIS (OSBECK, 1765)

作者：Lai, X. F.;Gao, H.;Lai, X. F.;Gao, H.;Kong, J.;Wang, Q. Y.

关键词：

Isolation and characterization of polymorphic microsatellite markers from Coilia ectenes

作者其他论文 更多>>

Effects of dietary supplementation of A3 alpha peptidoglycan on the growth, immune response and defence of sea cucumber

Genetic diversity of different populations and improved growth in the F1 hybrids in the swimming crab (Portunus trituberculatus)

Edwardsiellosis in farmed turbot, Scophthalmus maximus (L.), associated with an unusual variant of Edwardsiella tarda: a clinical, aetiological and histopathological study

Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes

CLONING AND CHARACTERIZATION OF A TRANSCRIPTIONAL REPRESSOR FROM FENNEROPENAEUS CHINENSIS (OSBECK, 1765)

A3 alpha-peptidoglycan extracted from Bifidobacterium sp could enhance immunological ability of Apostichopus japonicus

IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF A CHITIN BINDING-LIKE PROTEIN FROM FENNEROPENAEUS CHINENSIS (OSBECK, 1765)

意 见 箱

作者其他论文更多>>

意见箱