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Monochasma Savatieri Aqueous Extract inhibits Human Breast Cancer Cell Line Migration and Adhesion Without Generating Toxicity

文献类型： 外文期刊

作者： Tan, Lin 1 ; Solis-Sainz, Juan C. 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

2.Autonomous Univ Queretaro, Sch Med, Dept Biomed Res, Queretaro 76170, Qro, Mexico

关键词： Monochasma savatieri; breast cancer; cell migration; cell adhesion; cytotoxicity; acteoside

期刊名称：ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY （ 影响因子：2.8； 五年影响因子：2.7 ）

ISSN： 1871-5206

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Background Monochasma savatieri, is a rare and endangered plant used to treat cancer in Chinese traditional medicine. Objective To evaluate the anti-cancer activity of M. savatieri aqueous extract by determining its cytotoxicity, anti-migratory, and anti-adhesion effects on breast cancer cells. Methods Cell viability, migration, adhesion, circularity, and cell cycle were evaluated by crystal violet (CV) staining, wound-healing, and transwell assays and flow cytometry in MCF7 and MDA-MB-231 cells. Caveolin-1, snail, vimentin and activated Erk and Akt expression were determined by western blot in MDA-MB-231 cells. Immunofluorescent assays confirmed caveolin-1 expression in MDA-MB-231 cells. Results Survival and cell cycle of MCF7 and MDA-MB-231 cells were not modified by doses up to 500 mu g/mL of the extract. The extract inhibited cell migration and adhesion of MDA-MB-231 cells. When cells were exposed to the extract, there was a slight decrease in protein expression of factors related to epithelial-to-mesenchymal transition (snail and vimentin) and a strong decrease in the expression of the oncogenic membrane protein caveolin-1. Furthermore, the levels of phosphorylated Erk and Akt were also decreased. The content of acteoside, a phenylpropanoid glycoside with reported anti-cancer activity present in M. savatieri, was almost 5 times as much as isoacteoside. Conclusion M. savatieri possesses anti-cancer activity without exerting cytotoxicity on breast cancer cells. The extract exhibited anti-migratory and anti-adhesion effects on breast cancer cells by regulating Erk and Akt signaling pathways and the expression of caveolin-1. In addition, acteoside present in M. savatieri could be responsible for the observed effects.

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