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Validation of LC-MS/MS Coupled with a Chiral Column for the Determination of 3-or 15-Acetyl Deoxynivalenol Mycotoxins from Fusarium graminearum in Wheat

文献类型： 外文期刊

作者： Wang, Lan 1 ; Yan, Zheng 1 ; Zhou, Haiyan 1 ; Fan, Yingying 2 ; Wang, Cheng 2 ; Zhang, Jingbo 4 ; Liao, Yucai 4 ; Wu, Aib 1 ;

作者机构： 1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth, SIBS UGENT SJTU Joint Lab Mycotoxin Res,CAS Key L, Shanghai 200030, Peoples R China

2.Minist Agr & Rural Affiairs, Lab Qual & Safety Risk Assessment Agroprod Urumqi, Key Lab Agroprod Qual & Safety Xinjiang, Urumqi 830091, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Urumqi 830091, Peoples R China

4.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China

关键词： chromatographic separation; chemotype determination; mycotoxin; wheat

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN：

年卷期： 2021 年 13 卷 9 期

页码：

收录情况： SCI

摘要： The major causal agents Fusarium graminearum (F. graminearum) and Fusarium asiaticum could produce multiple mycotoxins in infected wheat, which threatens the health of humans and animals. Specifically, deoxynivalenol (DON) and its derivatives 3- and 15-acetyldeoxynivalenol (3-ADON and 15-ADON) are commonly detected mycotoxins in cereal grains. However, the good chromatographic separation of 3-ADON and 15-ADON remains challenging. Here, an LC-MS/MS method for the chemotype determination of Fusarium strains was developed and validated. 3- and 15-ADON could be separated chromatographically in this study with sufficiently low limits of detection (LODs; 4 mu g/kg) and limits of quantification (LOQs; 8 mu g/kg). The satisfying intraday and interday reproducibility (both %RSDr and %RSDR were <20%) of this method indicated good stability. The recoveries of all analytes were in the range of 80-120%. In addition, three F. graminearum complex (FGC) strains, i.e., PH-1 (chemotype 15-ADON), F-1 (chemotype 3-ADON) and 5035 (chemotype 15-ADON), were selected to verify the accuracy of the method in differentiating phenotypes. The validation results showed that this LC-MS/MS method based on sample pretreatment is effective and suitable for the chromatographic separation of 3-ADON and 15-ADON in wheat.

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