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MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families

文献类型： 外文期刊

作者： Du Min 1 ; Chen SongLin 1 ; Liu YanHong 2 ; Niu BaoZhen 2 ; Yang JingFeng 1 ; Zhang Bo 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Honghe Univ, Coll Life Sicence & Technol, Mengzi 661100, Peoples R China

3.Shanghai Ocean Univ, Coll Aqua Life Sci & Technol, Shanghai 200090, Peoples R China

关键词： turbot (Scophthalmus maximus);Edwardsiella tarda;major histocompatibility complex II B;polymorphism;resistance;susceptibility

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2012 年 57 卷 25 期

页码：

收录情况： SCI

摘要： This study examined genetic variation in the major histocompatibility complex (MHC) Class II B gene in turbot (Scophthalmus maximus) by virulent bacterial pathogen challenge. One hundred fry from each of six families were infected with Edwardsiella tarda by intraperitoneal injection. Family mortality ranged from 28.0% to 83.3%. Complete exon 2 and intron 1 sequences of MHC Class II B genes were amplified from five survivor and five non-survivor individuals per family using the clone-sequence method. Thirty-seven sequences from 60 individuals revealed 37 different alleles, 25 of which were unique to this study. The 25 unique alleles belonged to 16 major allele types. Nine alleles were used to examine the association between alleles and resistance/susceptibility to disease. Five alleles were present in an individual, suggesting a minimum of three loci or copies of the turbot MHC Class II B gene. The rate of non-synonymous substitution (d (N)) was 2.30 and 1.58 times higher than synonymous substitution (d (S)) in the peptide-binding regions (PBR) and non-PBR in whole families, respectively, which suggested balancing selection on exon 2 of the MHC Class II B gene in turbot. One allele, Scma-DBB1*02, was significantly more prevalent in survivor stock than in non-survivor stock (P=0.001). Therefore, this allele might be associated with resistance to bacteria. A second allele, Scma-DBB1*10, was significantly more prevalent in non-survivor stock (P=0.021), and is likely associated with susceptibility to bacteria.

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[1]Specific MHC class II B alleles associated with resistance to Edwardsiella tarda in turbot, Psetta maxima (L.). Xu, J-Y,Chen, S-L,Ding, H.,Xu, J-Y. 2009

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[15]A real-time PCR targeted to the upstream regions of HlyB for specific detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Xie Guosi,Huang Jie. 2012

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[20]Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain. Sun, Yun,Liu, Chun-sheng,Sun, Li,Sun, Yun,Liu, Chun-sheng. 2010

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