您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The role of tyrosine hydroxylase and dopa decarboxylase in melanin biosynthesis and metamorphosis in Coccinella septempunctata

文献类型： 外文期刊

作者： Chen, Xu 1 ; Zhang, Yu 3 ; Wu, Meng-meng 1 ; Huang, Yuan-Qin 1 ; Zang, Lian-sheng 1 ; Wang, Su 3 ; Xiao, Da 3 ;

作者机构： 1.Guizhou Univ, State Key Lab Green Pesticide, Guiyang 550025, Peoples R China

2.Guizhou Univ, Ctr R&D Fine Chem, Key Lab Green Pesticide & Agr Bioengn, Minist Educ, Guiyang, Peoples R China

3.Jilin Agr Univ, Inst Biol Control, Jilin Engn Res Ctr Resource Insects Industrializat, Changchun, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing, Peoples R China

5.Minist Agr & Rural Affairs, Key Lab Nat Enemies Insects, Beijing, Peoples R China

关键词： Melanin synthesis; seven-spotted ladybird beetle; dopamine; holometabolous; cuticle

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：4.6； 五年影响因子：4.4 ）

ISSN： 0171-8177

年卷期： 2024 年 44 卷 5 期

页码：

收录情况： SCI

摘要： Melanin has been recognized as a crucial factor in the evolution, polymorphisms, and environmental adaption of insects. Within the Coleoptera family, ladybird beetles exhibit remarkable phenotypic variation characterized by the number and size of black spots on their elytra. This distinctive characteristic makes ladybird as ideal models for studying melanin synthesis and its associated physiological effects. In this study, we focused on tyrosine hydroxylase (CsTH) and dopa decarboxylase (CsDDC), which participate in melanin biosynthesis in the monomorphic seven-spotted ladybird beetle, Coccinella septempunctata. Spatial expression profiles in adults revealed that CsTH was predominantly expressed in the elytra, while CsDDC was primarily expressed in elytra and ovaries. RNA interference (RNAi)-mediated silencing of these genes in fourth instar larvae led to reduced melanin on the cuticle, complete mortality shortly after eclosion, and disrupted eclosion processes. Furthermore, dopamine levels significantly decreased following the silencing of CsTH. Silencing CsTH during early pupal stages prevented eclosion, while later silencing permitted eclosion but resulted in melanin deficiency. In contrast, silencing CsDDC did not affect adult cuticle melanin synthesis. Our findings highlighted the vital roles of CsTH and CsDDC in melanin synthesis and metamorphosis in C. septempunctata, demonstrating that dopamine melanin is the primary component of cuticle melanin and underscoring the multifunctional effects of these genes on metamorphosis. The results highlight some mechanisms underneath insect melanization during cuticle formation and provide evidence of the multifunctional effects of essential genes in metamorphosis process in insects.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台