Integrative analysis of miRNA-mRNA expression in the brain during high temperature-induced masculinization of female Nile tilapia ( Oreochromis niloticus )
文献类型： 外文期刊
作者： Jiang, Bingjie 1 ; Lu, Siqi 1 ; Li, Yan 2 ; Badran, M. F. 3 ; Dong, Yalun 1 ; Xu, Pao 1 ; Qiang, Jun 1 ; Tao, Yifan 1 ;
作者机构： 1.Minist Agr & Rural Affairs, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resources, Chinese Acad Fishery Sci, Wuxi, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Jiangsu, Peoples R China
3.Suez Canal Univ, Fish Farming & Technol Inst, Aquat Hatchery Prod Dept, Ismailia, Egypt
4.9 Shanshui East Rd, Wuxi 214081, Peoples R China
关键词： Oreochromis niloticus; Brain tissues; High temperature -induced; Sex reversal; miRNA-mRNA expression
期刊名称：GENOMICS （ 影响因子：4.4； 五年影响因子：4.2 ）
ISSN： 0888-7543
年卷期： 2024 年 116 卷 3 期
页码：
收录情况： SCI
摘要： Temperature is one of the most important non -genetic sex differentiation factors for fish. The technique of high temperature -induced sex reversal is commonly used in Nile tilapia ( Oreochromis niloticus ) culture, although the molecular regulatory mechanisms involved in this process remain unclear. The brain is an essential organ for the regulation of neural signals involved in germ cell differentiation and gonad development. To investigate the regulatory roles of miRNAs-mRNAs in the conversion of female to male Nile tilapia gender under hightemperature stress, we compared RNA-Seq data from brain tissues between a control group (28 degrees C) and a high temperature -treated group (36 degrees C). The result showed that a total of 123,432,984 miRNA valid reads, 288,202,524 mRNA clean reads, 1128 miRNAs, and 32,918 mRNAs were obtained. Among them, there were 222 significant differentially expressed miRNAs (DE miRNAs) and 810 differentially expressed mRNAs (DE mRNAs) between the two groups. Eight DE miRNAs and eight DE mRNAs were randomly selected, and their expression patterns were validated by qRT-PCR. The miRNA-mRNA co -expression network demonstrated that 40 DE miRNAs targeted 136 protein -coding genes. Functional enrichment analysis demonstrated that these genes were involved in several gonadal differentiation pathways, including the oocyte meiosis signaling pathway, progesterone -mediated oocyte maturation signaling pathway, cell cycle signaling pathway and GnRH signaling pathway. Then, an interaction network was constructed for 8 miRNAs (mir-137-5p, let -7d, mir-1388-5p, mir124-4-5p, mir-1306, mir-99, mir-130b and mir-21) and 10 mRNAs ( smc1al , itpr2 , mapk1 , ints8 , cpeb1b , bub1 , fbxo5 , mmp14b , cdk1 and hrasb ) involved in the oocyte meiosis signaling pathway. These findings provide novel information about the mechanisms underlying miRNA-mediated sex reversal in female Nile tilapia.
- 相关文献
作者其他论文 更多>>
-
In Vitro Culture of Glochidia and Morphological Changes in Juveniles of the Endangered Freshwater Mussel Solenaia oleivora
作者：Ma, Xueyan;Jin, Wu;Lv, Guohua;Chen, Wanwen;Xu, Dongpo;Xu, Pao;Wen, Haibo;Ma, Xueyan;Jin, Wu;Lv, Guohua;Chen, Wanwen;Xu, Dongpo;Xu, Pao;Hua, Dan;Wen, Haibo;Jin, Wu;Xu, Dongpo;Xu, Pao;Wen, Haibo
关键词：glochidia; in vitro culture; juvenile; morphologic; Solenaia oleivora
-
Chromosome-level genome assembly of the freshwater mussel Sinosolenaia oleivora (Heude, 1877)
作者：Ma, Xueyan;Jin, Wu;Chen, Wanwen;Liu, Qian;Jiang, Haizhou;Zhou, Yanfeng;Xu, Pao;Wen, Haibo;Xu, Dongpo;Ma, Xueyan;Jin, Wu;Chen, Wanwen;Xu, Pao;Wen, Haibo;Xu, Dongpo;Jin, Wu;Liu, Qian;Jiang, Haizhou;Zhou, Yanfeng;Xu, Pao;Wen, Haibo;Xu, Dongpo
关键词：
-
Molecular characterization and function of hif1a and fih1 in response to acute thermal stress in American shad (Alosa sapidissima)
作者：Liang, Zhengyuan;Hu, Songqin;Miao, Linghong;Zhu, Wenbin;Wang, Lanmei;Dong, Zaije;Liang, Zhengyuan;Dong, Yalun;Miao, Linghong;Zhu, Wenbin;Fu, Jianjun;Luo, Mingkun;Wang, Lanmei;Dong, Zaije;Dong, Yalun;Miao, Linghong;Zhu, Wenbin;Fu, Jianjun;Luo, Mingkun;Wang, Lanmei;Dong, Zaije;Feng, Bingbing
关键词：Alosa sapidissima; Hypoxia-inducible factor-1 alpha; Factor inhibiting hif1 alpha; Thermal stress; Housekeeping genes selection
-
Integrative analysis of metagenome and metabolome provides new insights into intestinal health protection in Coilia nasus larvae via probiotic intervention
作者：Mang, Qi;Xu, Gangchun;Xu, Pao;Mang, Qi;Gao, Jun;Li, Quanjie;Sun, Yi;Xu, Gangchun;Xu, Pao
关键词：Coilia nasus; Lactiplantibacillus plantarum; Gut microbiota; Metabolite; Growth performance; Intestinal health
-
Silencing the fatty acid elongase gene elovl6 induces reprogramming of nutrient metabolism in male Oreochromis niloticus
作者：Tao, Yi-Fan;Zhong, Chun-Yi;Hua, Ji-Xiang;Lu, Si-Qi;Li, Yan;Dong, Ya-Lun;Xu, Pao;Jiang, Bing-Jie;Qiang, Jun;Tao, Yi-Fan;Pan, Yi-Fan;Wang, Qing-Chun;Xu, Pao;Qiang, Jun;Qiang, Jun
关键词：Oreochromis niloticus elovl6; Antisense RNA; Nutrient metabolism; Regulatory mechanism
-
Geographical traceability of flavor compounds in Chinese mitten crab (Eriocheir sinensis): Implications for quality differentiation, authenticity assessment, and mechanism research
作者：Li, Jiayi;Nie, Zhijuan;Hu, Jiawen;Wang, Lanmei;Song, Chao;Xu, Dongpo;Gao, Jiangcao;Xu, Pao;Xu, Gangchun;Nie, Zhijuan;Xu, Gangchun;Xu, Gangchun
关键词：Eriocheir sinensis; Volatile organic compounds; Geographical origin; Elemental analysis; Interaction analysis
-
Aquaculture in Zambia: The Current Status, Challenges, Opportunities and Adaptable Lessons Learnt from China
作者：Zhang, Lin;Hua, Fenglei;Xu, Pao;Zhang, Lin;Hua, Fenglei;Xu, Pao;Maulu, Sahya;Maulu, Sahya;Chama, Majory K. H.
关键词：Zambia; aquaculture; challenges; adaptable lessons
