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The Application of DNA Barcoding in Crustacean Larvae Identification from the Zhongsha Islands, South China

文献类型： 外文期刊

作者： Xu, Lei 1 ; Wang, Xuehui 1 ; Huang, Delian 1 ; Wang, Lianggen 1 ; Ning, Jiajia 1 ; Li, Yafang 1 ; Liu, Shuangshuang 1 ; Du, Feiyan 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Peoples R China

2.Guangdong Prov Key Lab Fishery Ecol & Environm, Guangzhou, Peoples R China

关键词： DNA identification; COI gene; crustacean larvae; coral reef; Zhongsha Islands

期刊名称：FRONTIERS IN MARINE SCIENCE （ 影响因子：5.247； 五年影响因子：5.72 ）

ISSN：

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： Marine crustaceans are known as a group with high morphological diversity and great economic value. Most species have planktonic larval stages that are difficult to identify to species level using traditional approaches because of insufficient morphological diagnostic characters or taxonomic descriptions. We used DNA barcoding and molecular species identification to investigate the species diversity and distribution of crustacean larvae in the Zhongsha Islands waters, South China Sea. In total, 108 sequences were obtained from crustacean larvae collected in the Zhongsha Islands waters in 2019 using vertical hauls between the depths of 5 and 200 m. The molecular classification approach confirmed that 108 sequences represented crustaceans typical to the South China Sea, with 70 species identified, representing 43 genera, 23 families, and 4 orders. However, the cytochrome c oxidase subunit I gene sequences of only 27 species identified from the larval samples matched with available sequences taken from adults in GenBank. The comparison of K2P distances yielded a notable gap of 3.5-10.7% between intraspecific and interspecific distances across the sequence dataset. More than 80% of the crustacean larvae species belonged to the order Decapoda, and they displayed marked differences in their distributionsin the Zhongshan Islands waters. The orders Calanoida and Amphipoda were represented by the fewest species, which were recorded only at the edge of the Zhongsha Atoll.

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