您好,欢迎访问中国水产科学研究院 机构知识库!

Evaluation of putative internal reference genes for gene expression normalization in Nannochloropsis sp by quantitative real-time RT-PCR

文献类型: 外文期刊

作者: Cao, Shaona 1 ; Zhang, Xiaowen 1 ; Ye, Naihao 1 ; Fan, Xiao 1 ; Mou, Shanli 1 ; Xu, Dong 1 ; Liang, Chengwei 3 ; Wang, Yi 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Qingdao Agr Univ, Qingdao 266109, Peoples R China

3.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China

关键词: Nannochloropsis sp.;Gene expression;Normalization;RT-qPCR;Reference genes

期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )

ISSN: 0006-291X

年卷期: 2012 年 424 卷 1 期

页码:

收录情况: SCI

摘要: Quantitative real-time reverse transcription PCR (RT-qPCR), a sensitive technique for quantifying gene expression, depends on the stability of the reference gene(s) used for data normalization. To date, few studies on reference genes have been undertaken for Nannochloropsis sp. In this study, 12 potential reference genes were evaluated for their expression stability using the geNorm and NormFinder statistical algorithms by RT-qPCR. The results showed that the best reference genes differed depending on the treatments: different light intensities (DL), the diurnal cycle (DC), high light intensity (HL) and low temperature treatments (LT). A combination of ACT1, ACT2 and TUA would be appropriate as a reference panel for normalizing gene expression data across all the treatments. ACT2 showed the most stable expression across all tested samples but was not the most stable one for individual treatments. Though 18S showed the least stable expression considering all tested samples, it is the most stable one for LT using geNorm. The expression of Lhc confirmed that the appropriate reference genes are crucial. These results provide a foundation for more accurate use of RT-qPCR under different experimental conditions in Nannochloropsis sp. gene analysis. (C) 2012 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Reference genes for gene expression normalization in Chlamydomonas sp ICE-L by quantitative real-time RT-PCR. Mou, Shanli,Zhang, Xiaowen,Xu, Dong,Ye, Naihao,Miao, Jinlai,Zheng, Zhou.

[2]Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus). Yang, Chang Geng,Tian, Juan,Liu, Wei,Wu, Fan,Jiang, Ming,Wen, Hua,Wang, Xian Li.

[3]The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza. Zhang, Xiaowen,Xu, Dong,Ye, Naihao,Dong, Meitao,Wang, Wenqi,Chi, Xiaoyuan,Mou, Shanli,Xu, Jianfang.

[4]De Novo Sequencing and Global Transcriptome Analysis of Nannochloropsis sp (Eustigmatophyceae) Following Nitrogen Starvation. Liang, Chengwei,Su, Zhongliang,Cao, Shaona,Zhang, Xiaowen,Xu, Dong,Ye, Naihao,Zhu, Baohua,Guang, Xiangyu. 2013

[5]半滑舌鳎gata5基因的克隆及表达分析. 董忠典,张宁,崔忠凯,徐文腾,陈松林. 2019

[6]Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase. Rahman, N. M. A.,Fu, H. T.,Jin, S.,Bai, H. K.,Liang, G. X.,Gong, Y. S.,Wu, Y.,Fu, H. T.,Sun, S. M.,Qiao, H.,Jin, S.,Zhang, W. Y.,Xiong, Y. W.,Rahman, N. M. A.. 2016

[7]Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense. Rahman, N. M. A.,Fu, H.,Bai, H.,Jiang, F. W.,Liang, G.,Jiang, F. F.,Wu, Y.,Fu, H.,Qiao, H.,Jin, S.,Zhang, W.,Sun, S.,Gong, Y.,Xiong, Y.,Rahman, N. M. A.. 2016

[8]Identification and comparative analysis of the Pseudosciaena crocea microRNA transcriptome response to poly(I:C) infection using a deep sequencing approach. Qi, Pengzhi,Guo, Baoying,Zhu, Aiyi,Wu, Changwen,Liu, Changlin.

[9]Characterization, expression and silencing by RNAi of p53 from Penaeus monodon. Dai, Wenting,Qiu, Lihua,Zhao, Chao,Fu, Mingjun,Ma, Zhenhua,Zhou, Falin,Yang, Qibin,Qiu, Lihua,Zhao, Chao,Fu, Mingjun,Ma, Zhenhua,Zhou, Falin,Yang, Qibin,Dai, Wenting,Yang, Qibin.

[10]Cloning, expression and methylation analysis of piwil2 in half-smooth tongue sole (Cynoglossus semilaevis). Zhang, Liyan,Zhao, Wen,Zhang, Liyan,Liu, Wanjun,Shao, Changwei,Zhang, Ning,Li, Hailong,Liu, Kun,Dong, Zhongdian,Qi, Qian,Chen, Songlin,Liu, Wanjun,Dong, Zhongdian,Qi, Qian.

[11]Participation of calmodulin in ovarian maturation induced by eyestalk ablation in red swamp crayfish Procambarus clarkii. Guan, Zheng-Bing,Song, Chen-Meng,Liao, Xiang-Ru,Shui, Yan,Zhou, Xin,Xu, Zeng-Hong,Zhao, Chao-Yang,Zhou, Xin.

[12]Cloning, characterization, expression analysis and RNAi of Retinoblastoma-like gene from black tiger shrimp (Penaeus monodon). Xie, Bobo,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Xie, Bobo,Xie, Bobo,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Qiu, Lihua.

[13]Functional characteristic and differential expression of myostatin in Chlamys nobilis. Zhu, Kecheng,Guo, Huayang,Zhang, Nan,Li, Yundong,Jiang, Shigui,Zhang, Dianchang. 2017

[14]Fasted and postprandial response of serum physiological response, hepatic antioxidant abilities and HSP70 expression in Wuchang bream (Megalobrama amblycephala) fed different dietary carbohydrate levels. Zhou, Chuanpeng,Ge, Xianping,Liu, Bo,Xie, Jun,Chen, Ruli,Zhou, Chuanpeng. 2014

[15]Molecular Cloning and Response to Water Temperature and Nutrient Manipulation of Insulin-like Growth Factor (IGF) Genes in Golden Pompano Trachinotus ovatus (Linnaeus 1758) Larvae. Ma, Zhenhua,Hu, Jing,Liu, Yajuan,Yang, Rui,Sun, Dianrong,Ma, Zhenhua,Sun, Dianrong,Ma, Zhenhua,Qin, Jian G.. 2016

[16]Physiological responses and gene expression changes in the western mosquitofish (Gambusia affinis) exposed to progesterone at environmentally relevant concentrations. Hou, Liping,Yang, Yang,Shu, Hu,Cheng, Xuemei,Xu, Hongyan,Ying, Guangguo,Zhao, Jianliang,Ying, Guangguo,Zhao, Jianliang. 2017

[17]Molecular characterization, expression and activity of glucokinase in golden pompano, Trachinotus ovatus: Response of its expression to carbohydrate in the diet. Zhou, Chuanpeng,Wang, Jun,Wang, Yun,Lin, Heizhao,Huang, Zhong,Yu, Wei. 2018

[18]Expression profiles of NODs in channel catfish (Ictalurus punctatus) after infection with Edwardsiella tarda, Aeromonas hydrophila, Streptococcus iniae and channel catfish hemorrhage reovirus. Li, Min,Wang, Qi-long,Lu, Yang,Chen, Song-lin,Sha, Zhen-xia,Li, Min,Li, Qi,Lu, Yang. 2012

[19]Identification of a cobia (Rachycentron canadum) CC chemokine gene and its involvement in the inflammatory response. Feng, Juan. 2012

[20]Identification and mRNA expression of antioxidant enzyme genes associated with the oxidative stress response in the Wuchang bream (Megalobrama amblycephala Yih) in response to acute nitrite exposure. Sun, Shengming,Ge, Xianping,Zhu, Jian,Xuan, Fujun,Jiang, Xiaojun. 2014

作者其他论文 更多>>