Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho
文献类型: 外文期刊
作者: Liu, Shufang 1 ; Liu, Hongbo 1 ; Lin, Lin 3 ; Yuan, Yanjiao 1 ; Dai, Fangqun 1 ; Zhuang, Zhimeng 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Key Lab Fishery Resources & Ecoenvironm, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture, Dalian 116023, Peoples R China
3.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词: Solenocera crassicornis;Solenocera melantho;microsatellite marker;cross-species amplification
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )
ISSN: 1422-0067
年卷期: 2012 年 13 卷 7 期
页码:
收录情况: SCI
摘要: Twenty microsatellite markers were isolated and characterized for Solenocera crassicornis from a (GT)(13)-enriched genomic library. Their polymorphisms were investigated using 44 wild individuals from the South Yellow Sea. Our investigation revealed that all the markers were polymorphic. The number of alleles per locus varied from 6 to 19 with an average of 12.35. The observed and expected heterozygosities ranged from 0.400 to 0.977 and from 0.609 to 0.940, with averages of 0.788 and 0.859, respectively. Four loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni's correction. Cross-species amplification was also conducted in Solenocera melantho collected from the East China Sea. The result showed that 14 loci could be amplified from Solenocera melantho DNAs. These polymorphic markers would be useful for assessment of genetic variation and population structure of S. crassicornis and S. melantho.
- 相关文献
作者其他论文 更多>>
-
Identification of "Bathed" Chinese Mitten Crabs (Eriocheir sinensis) Using Geometric Morphological Analysis of the Carapace
作者:Xu, Yiqian;Liu, Hongbo;Yang, Jian;Xue, Junren;Liu, Hongbo;Jiang, Tao;Chen, Xiubao;Yang, Jian
关键词:Eriocheir sinensis; landmark; geometric morphometrics; "introduced"/"bathed" crab; discriminant analysis; aquaculture
-
Reconstruction of Historical Metal Backgrounds in Lacustrine Environments of China Using an Anodonta woodiana "Specimen Bank"
作者:Yan, Mingjun;Yang, Jian;Chen, Xiubao;Xue, Junren;Liu, Hongbo;Yang, Jian
关键词:Specimen bank; Freshwater mussel; Element; Lake; Monitoring
-
Integrative Metabolomics, Enzymatic Activity, and Gene Expression Analysis Provide Insights into the Metabolic Profile Differences between the Slow-Twitch Muscle and Fast-Twitch Muscle of Pseudocaranx dentex
作者:Wang, Huan;Li, Busu;Li, Ang;An, Changting;Liu, Shufang;Zhuang, Zhimeng;Li, Busu;Liu, Shufang
关键词:Pseudocaranx dentex; slow-twitch muscle; fast-twitch muscle; untargeted metabolomics; energy metabolism; regulatory mechanism
-
DNA barcoding, identification, and validation of the pufferfish (Order: Tetraodontiformes) in China coastal waters
作者:Liu, Kaiying;Sun, Hongyue;Zhao, Xin;Wang, Chaoqun;An, Changting;Li, Ang;Liu, Shufang;Zhuang, Zhimeng;Liu, Kaiying;Wang, Chaoqun;Zhao, Xin;Li, Ang;Liu, Shufang;Liu, Shufang
关键词:DNA barcoding; morphological characteristics; phylogeny; species identification; synonym; Tetraodontiformes
-
Mitochondrial Homeostasis Regulating Mitochondrial Number and Morphology Is a Distinguishing Feature of Skeletal Muscle Fiber Types in Marine Teleosts
作者:Li, Busu;Wang, Huan;Zeng, Xianghui;Liu, Shufang;Zhuang, Zhimeng;Li, Busu;Liu, Shufang
关键词:Takifugu rubripes; slow-twitch muscles; fast-twitch muscles; mitochondrial homeostasis; mitochondrial adaptation
-
Copper Stress Causes Shell Morphology Changes in Early Juvenile Anodonta woodiana Based on Geometric-Morphometric Analysis
作者:Yan, Mingjun;Yang, Jian;Chen, Xiubao;Xue, Junren;Liu, Hongbo;Jiang, Tao;Yang, Jian
关键词:Copper; Stress; Chinese pond mussel Anodonta woodiana; Shell shape; Geometric-morphometric; Non-lethal assessment
-
Stable isotopic fingerprints of genuine and "bathing" cultured Chinese mitten crabs (Eriocheir sinensis) in Yangcheng Lake, China
作者:Xue, Junren;Yang, Jian;Jiang, Tao;Chen, Xiubao;Liu, Hongbo;Yang, Jian
关键词:Authentication; Adulteration; Yangcheng Lake; Eriocheir sinensis; "Bath" culture; Stable isotopes