Functional validation of pathogenicity genes in rice sheath blight pathogen Rhizoctonia solani by a novel host-induced gene silencing system
文献类型： 外文期刊
作者： Zhao, Mei 1 ; Wang, Chenjiaozi 1 ; Wan, Jun 1 ; Li, Zanfeng 1 ; Liu, Dilin 2 ; Yamamoto, Naoki 3 ; Zhou, Erxun 1 ; Shu, C 1 ;
作者机构： 1.South China Agr Univ, Dept Plant Pathol, Guangdong Prov Key Lab Microbial Signals & Dis Co, Guangzhou 510642, Peoples R China
2.Guangdong Prov Key Lab New Technol Rice Breeding, Guangzhou, Peoples R China
3.Sichuan Agr Univ, Coll Agron, Chengdu, Peoples R China
关键词： rice disease control strategy; host-induced gene silencing; pathogenicity gene; Rhizoctonia solani; rice sheath blight
期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）
ISSN： 1464-6722
年卷期： 2021 年 22 卷 12 期
页码：
收录情况： SCI
摘要： Rice sheath blight, caused by the soilborne fungus Rhizoctonia solani, causes severe yield losses worldwide. Elucidation of the pathogenic mechanism of R. solani is highly desired. However, the lack of a stable genetic transformation system has made it challenging to examine genes' functions in this fungus. Here, we present functional validation of pathogenicity genes in the rice sheath blight pathogen R. solani by a newly established tobacco rattle virus (TRV)-host-induced gene silencing (HIGS) system using the virulent R. solani AG-1 IA strain GD-118. RNA interference constructs of 33 candidate pathogenicity genes were infiltrated into Nicotiana benthamiana leaves with the TRV-HIGS system. Of these constructs, 29 resulted in a significant reduction in necrosis caused by GD-118 infection. For further validation of one of the positive genes, trehalose-6-phosphate phosphatase (Rstps2), stable rice transformants harbouring the double-stranded RNA (dsRNA) construct for Rstps2 were created. The transformants exhibited reduced gene expression of Rstps2, virulence, and trehalose accumulation in GD-118. We showed that the dsRNA for Rstps2 was taken up by GD-118 mycelia and sclerotial differentiation of GD-118 was inhibited. These findings offer gene identification opportunities for the rice sheath blight pathogen and a theoretical basis for controlling this disease by spray-induced gene silencing.
- 相关文献
作者其他论文 更多>>
-
Characterization of a novel gammapartitivirus infecting the phytopathogenic fungus Pyricularia oryzae
作者：Fan, Yu;Zhao, Wenhua;Tang, Xiaolin;Wang, Li;Yang, Mei;Zhou, Erxun;He, Zhenrui;Yang, Yingqing;Cheng, Baoping
关键词：
-
Co-infection of Four Novel Mycoviruses from Three Lineages Confers Hypovirulence on Phytopathogenic Fungus Ustilaginoidea virens
作者：Fan, Yu;Zhao, Wenhua;Tang, Xiaolin;Yang, Mei;Zhang, Zixuan;Zhou, Erxun;He, Zhenrui;Yang, Yingqing;Cheng, Baoping
关键词：Mycovirus; Rice false smut; Co-infection; Biocontrol
-
Dominance complementation of parental heading date alleles of Hd1, Ghd7, DTH8, and PRR37 confers transgressive late maturation in hybrid rice
作者：Zong, Wubei;Song, Yingang;Xiao, Dongdong;Guo, Xiaotong;Li, Fuquan;Sun, Kangli;Tang, Wenjing;Xie, Wenhao;Luo, Yanqiu;Liang, Shan;Zhou, Jingyao;Xie, Xianrong;Chen, Letian;Wang, Haiyang;Liu, Yao-Guang;Guo, Jingxin;Liu, Dilin
关键词：hybrid rice; transgressive late maturation; photoperiod sensitivity; genetic basis; marker-assisted selection
-
Transcriptomic and methylomic analyses provide insights into the molecular mechanism and prediction of heterosis in rice
作者：Fu, Chongyun;Zhu, Manshan;Liu, Wuge;Ma, Xiaozhi;Li, Jinhua;Liao, Yilong;Liu, Dilin;Wang, Feng;Fu, Chongyun;Zhu, Manshan;Liu, Wuge;Ma, Xiaozhi;Li, Jinhua;Liao, Yilong;Liu, Dilin;Wang, Feng;Fu, Chongyun;Zhu, Manshan;Liu, Wuge;Ma, Xiaozhi;Li, Jinhua;Liao, Yilong;Liu, Dilin;Wang, Feng;Fu, Chongyun;Zhu, Manshan;Liu, Wuge;Ma, Xiaozhi;Li, Jinhua;Liao, Yilong;Liu, Dilin;Wang, Feng;Ma, Ce;Gu, Xiaofeng;Wang, Haiyang
关键词：Indica rice; heterosis; prediction; transcriptome; methylome
-
OsNRAMP2 facilitates Cd efflux from vacuoles and contributes to the difference in grain Cd accumulation between japonica and indica rice
作者：Yang, Wu;Chen, Luo;Ma, Yamei;Hu, Rui;Wang, Jian;Li, Wenhui;Dong, Jingfang;Yang, Tifeng;Zhou, Lian;Chen, Jiansong;Liu, Dilin;Yu, Ning;Liu, Zhixia;Zhang, Shaohong;Zhao, Junliang;Liu, Bin;Chen, Luo;Zhou, Lingyan
关键词：Rice(Oryza sativa L; ); Cadmium; OsNRAMP2; Haplotype
-
Bulk segregant analysis coupled with transcriptomics and metabolomics revealed key regulators of bacterial leaf blight resistance in rice
作者：Ma, Xiaozhi;Zhu, Manshan;Liu, Wuge;Li, Jinhua;Liao, Yilong;Liu, Dilin;Jin, Mengya;Fu, Chongyun;Wang, Feng;Ma, Xiaozhi;Zhu, Manshan;Liu, Wuge;Li, Jinhua;Liao, Yilong;Liu, Dilin;Jin, Mengya;Fu, Chongyun;Wang, Feng;Ma, Xiaozhi;Zhu, Manshan;Liu, Wuge;Li, Jinhua;Liao, Yilong;Liu, Dilin;Jin, Mengya;Fu, Chongyun;Wang, Feng
关键词：Bacterial leaf blight; Rice; Disease resistance; Genetics; R genes
-
Genome-wide association mapping and gene expression analysis reveal candidate genes for grain chalkiness in rice
作者：Huo, Xing;Wang, Jian;Chen, Luo;Fu, Hua;Yang, Tifeng;Dong, Jingfang;Ma, Yamei;Zhou, Lian;Chen, Jiansong;Liu, Dilin;Liu, Bin;Zhao, Junliang;Zhang, Shaohong;Yang, Wu
关键词：rice; grain chalkiness; genome-wide association study; quantitative trait loci (QTL); candidate gene
