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Comparison of in vitro strategies for predicting Dichlorodiphenyltrichloroethane (DDT) and its metabolites bioavailability from soils

文献类型： 外文期刊

作者： Li, Chao 1 ; Xu, Shen 3 ; Guan, Dong-xing 4 ; Chen, Xianxian 1 ; He, Huan 1 ;

作者机构： 1.Nanjing Normal Univ, Sch Environm, Nanjing 210023, Peoples R China

2.Nanjing Normal Univ, Sch Geog, Nanjing 210023, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjing 210014, Peoples R China

4.Zhejiang Univ, Inst Soil & Water Resources & Environm Sci, Coll Environm & Resource Sci, Hangzhou 310058, Peoples R China

关键词： In vitro assays; DDT; Mouse model; Tenax; Bioaccessibility; Bioavailability

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2023 年 256 卷

页码：

收录情况： SCI

摘要： In vitro strategies have widely been used to assess bioaccessibility of organic pollutants in soils. However, studies for comparing in vitro models with in vivo data are still limited. In this study, Dichlorodiphenyltrichloroethane (DDT) and its metabolites (called as DDTr) bioaccessibility in nine contaminated soils were measured using physiologically based extraction test (PBET), in vitro digestion model (IVD), and Deutsches Institut fur Normung (DIN) with/without Tenax as an absorptive sink, and DDTr bioavailability was assessed using an in vivo mouse model. Whether or not Tenax was added, DDTr bioaccessibility significantly varied among three methods, suggesting that DDTr bioaccessibility depended on the in vitro method employed. Multiple linear regression analysis indicated that sink, intestinal incubation time and bile content are identified to be the dominant factors in controlling DDTr bioaccessibility. Comparison of in vitro and in vivo results demonstrated that DIN assay with Tenax (TI-DIN) provided the best prediction for DDTr bioavailability (r(2)=0.66, slope=0.78). After extending intestinal incubation time to 6 h or increasing bile content to 4.5 g/L (same to DIN assay) of the TI-PBET and TIIVD assays, the in vivo-in vitro correlation will improved significantly, with r(2)=0.76 and slope=1.4 for TI-PBET and r(2)=0.84 and slope=1.9 for TI-IVD under 6 h intestinal incubation, and r(2)=0.59 and slope=0.96 for TIPBET and r(2)=0.51 and slope=1.0 for TI-IVD under 4.5 g/L of bile content. The results suggest that it is essential to understand these key factors influencing bioaccessibility for the development of standardized in vitro methods, which helps to refine the risk assessment of human exposure to contaminants via soil ingestion.

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