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Dual role of IGF-II in oocyte maturation in southern flounder Paralichthys lethostigma: Up-regulation of mPR alpha and resumption of meiosis

文献类型： 外文期刊

作者： Picha, Matthew E. 1 ; Shi, Bao 1 ; Thomas, Peter 1 ;

作者机构： 1.Univ Texas Austin, Inst Marine Sci, Port Aransas, TX 78373 USA

2.Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： Membrane progestin receptor;Insulin-like growth factor;Oocyte maturation;Southern flounder

期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）

ISSN： 0016-6480

年卷期： 2012 年 177 卷 2 期

页码：

收录情况： SCI

摘要： Increasing evidence suggests a regulatory role for the IGF system in teleost oocyte maturation (OM). Our objectives were to determine if IGF-I and IGF-II regulate different stages of OM in southern flounder (Paralichthys lethostigma) and to identify the likely maturation-inducing steroid (MIS) in this species. The most abundant final product of ovarian steroidogenesis assays eluted at the position of 17,20 beta,21-trihydroxy-4-pregnen-3-one (20 beta-S). 20 beta-S was also more potent in inducing germinal vesicle breakdown (GVBD) of maturationally-competent oocytes than other teleost MISs. IGF-II (100 nM) induced maturational competence (OMC), as greater GVBD was induced after incubation with IGF-II + 20 beta-S compared to that of the 20 beta-S + 20 beta-S or IGF-II + no treatment group. Incubation with IGF-II (100 nM) for 4-8 h significantly increased ovarian membrane progestin receptor alpha (mPR alpha or Paqr7b) mRNA levels 12-15% and mPR alpha protein levels 75-101%. Further, the IGF-II-induced increase in mPR alpha protein concentrations was partially blocked by pretreatment with Wortmannin, a Pik3 inhibitor, and PD 098,059, a Mapk inhibitor. Both IGF-I and -II (100 nM) induced GVBD of maturationally-competent oocytes was blocked by incubation with cycloheximide. Incubation with D,L-Aminoglutethimide decreased IGF-II-induced GVBD but had no effect on IGF-I-induced GVBD. IGF-I and -II were also able to induce GVBD of maturationally-incompetent oocytes, and elicited 75% and 135% greater GVBD, respectively, than hCG + 20 beta-S at 100 nM. In conclusion, we show that 20 beta-S is the likely MIS in this species and that IGF-I and -II are also able to induce GVBD. Further, IGF-II not only induces OMC but also up-regulates ovarian mPR alpha mRNA and protein through Pik3- and Mapk-dependent pathways. This is the first demonstration of mPR alpha regulation by an IGF in any vertebrate species. (c) 2012 Elsevier Inc. All rights reserved.

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[1]Establishment, characterization and virus susceptibility of a kidney-derived cell line from southern flounder, Paralichthys lethostigma Jordan & Gilbert. Wang, N.,Wang, X-L,Sha, Z-X,Tian, Y-S,Chen, S-L,Chen, S-L. 2011

[2]Role of animal pole protuberance and microtubules during meiosis in sea cucumber Apostichopus japonicus oocytes. Chang Yaqing,Yu Jiaping,Sun Huiling,Pang Zhenguo,Pang Zhenguo. 2010

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