EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica
文献类型: 外文期刊
作者: Liu, Fuli 1 ; Wang, Feijiu 1 ; Duan, Delin 2 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
关键词: Saccharina japonica;Laminaria digitata;EST;SSR;Phaeophyta
期刊名称:JOURNAL OF APPLIED PHYCOLOGY ( 影响因子:3.215; 五年影响因子:3.612 )
ISSN: 0921-8971
年卷期: 2012 年 24 卷 3 期
页码:
收录情况: SCI
摘要: The public availability of numerous expressed sequence tag (EST) enables EST-based SSR (simple sequence repeat) markers to be widely used for genetics and breeding studies. In the present study, EST-SSR markers were developed from ESTs of and were transferred to the non-congeneric species . Among the 2,668 non-redundant ESTs, 83 (3.1%) ESTs containing SSR were identified totally, with an average of one SSR per 13.6 kb. Analysis of SSR motifs revealed that the trinucleotide and tetranucleotide were major motifs, accounted for 44.58% and 16.87%, respectively. Based on the 83 ESTs containing SSR, we designed 45 pairs of primers in the flanking regions of the SSR, of which 13 pairs showed polymorphism in a wild population, and the mean alleles per locus was 3.6 (ranging from 2 to 6). The observed (Ho) and expected (He) heterozygosities of these EST-SSRs were 0.234-0.632 and 0.260-0.635, respectively. All loci were in Hardy-Weinberg equilibrium in the wild population and no linkage disequilibrium was detected among loci. The obtained EST-SSR markers can facilitate and promote related research such as ecological investigation, genetic diversity assessment and breeding practice of as well.
- 相关文献
作者其他论文 更多>>
-
Evaluation of heterosis and high temperature tolerance in hybrid offspring of different local Saccharina japonica cultivars
作者:Song, Guiwen;Yan, Yu;Huang, Xiang;Jin, Haiping;Xu, Yuanyuan;Liu, Fuli;Liu, Fuli;Zhang, Di
关键词:
-
Chronological development of the morphological, physiological, biochemical, and transcriptomic changes provides insights into the mechanisms of gametogenesis in Saccharina japonica
作者:Liang, Zhourui;Wang, Xiao;Zhang, Pengyan;Wang, Wenjun;Liu, Fuli;Liang, Zhourui;Zhang, Pengyan;Liu, Wenhui;Wang, Wenjun;Liu, Fuli
关键词:Saccharina japonica; Phaeophyceae; Gametogenesis; Physiology; Biochemistry; Ultrastructure; Transcriptome
-
Sorus developmental biology of hybrid cultivar in Saccharina japonica: Environmental and endogenous regulation
作者:Liu, Yi;Liu, Fuli;Liang, Zhourui;Zhang, Pengyan;Liu, Fuli;Liang, Zhourui;Zhang, Pengyan;Yuan, Yanmin;Wu, Yukun;Zhang, Di;Duan, Maohong
关键词:Saccharina japonica; Hybrid; Sorus; Seaweed cultivation; Sporogenesis
-
Regulation of Reproduction in Delayed Gametophyte of Saccharina japonica (Laminariales, Phaeophyceae): Effects of Light Intensity, Quality and Photoperiod
作者:Wang, Xiao;Liang, Zhourui;Zhang, Pengyan;Yuan, Yanmin;Liu, Yi;Wang, Wenjun;Sun, Xiutao;Liu, Fuli
关键词:Saccharina japonica; light intensity; light quality; photoperiod; gametogenesis; vegetative growth
-
The global dynamic of DNA methylation in response to heat stress revealed epigenetic mechanism of heat acclimation in Saccharina japonica
作者:Liu, Fuli;Liu, Fuli;Zhang, Pengyan;Liang, Zhourui;Yuan, Yanmin;Liu, Yi;Wu, Yukun;Zhang, Pengyan;Liang, Zhourui;Yuan, Yanmin;Liu, Yi;Wu, Yukun
关键词:differentially expressed gene; DNA methylation; heat stress; RNA-seq; Saccharina japonica; whole-genome bisulfite sequencing
-
Isolation and characterization of glutathione S-transferase genes and their transcripts in Saccharina japonica (Laminariales, Phaeophyceae) during development and under abiotic stress
作者:Lu, Chang;Li, Shuang;Cheng, Mengzhen;Duan, Delin;Lu, Chang;Zhang, Pengyan;Li, Shuang;Cheng, Mengzhen;Zhang, Pengyan;Li, Shuang;Cheng, Mengzhen
关键词:Saccharina japonica; Glutathione S-transferase; Transcriptome; Gene expression; Abiotic stresses; Enzyme activity assay
-
Genome-Wide Identification and Analysis of the Cryptochrome/Photolyase Family in the Brown Alga Saccharina japonica
作者:Wu, Yukun;Zhang, Pengyan;Liang, Zhourui;Yuan, Yanmin;Zhang, Di;Liu, Wenhui;Zhang, Di;Liu, Yi;Liu, Fuli
关键词:Cryptochrome; Photolyase Family (CPF); Blue-light photoreceptor; Genome-wide analysis; Saccharina japonica; Phaeophyceae; Gametophyte development