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EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica

文献类型: 外文期刊

作者: Liu, Fuli 1 ; Wang, Feijiu 1 ; Duan, Delin 2 ;

作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

关键词: Saccharina japonica;Laminaria digitata;EST;SSR;Phaeophyta

期刊名称:JOURNAL OF APPLIED PHYCOLOGY ( 影响因子:3.215; 五年影响因子:3.612 )

ISSN: 0921-8971

年卷期: 2012 年 24 卷 3 期

页码:

收录情况: SCI

摘要: The public availability of numerous expressed sequence tag (EST) enables EST-based SSR (simple sequence repeat) markers to be widely used for genetics and breeding studies. In the present study, EST-SSR markers were developed from ESTs of and were transferred to the non-congeneric species . Among the 2,668 non-redundant ESTs, 83 (3.1%) ESTs containing SSR were identified totally, with an average of one SSR per 13.6 kb. Analysis of SSR motifs revealed that the trinucleotide and tetranucleotide were major motifs, accounted for 44.58% and 16.87%, respectively. Based on the 83 ESTs containing SSR, we designed 45 pairs of primers in the flanking regions of the SSR, of which 13 pairs showed polymorphism in a wild population, and the mean alleles per locus was 3.6 (ranging from 2 to 6). The observed (Ho) and expected (He) heterozygosities of these EST-SSRs were 0.234-0.632 and 0.260-0.635, respectively. All loci were in Hardy-Weinberg equilibrium in the wild population and no linkage disequilibrium was detected among loci. The obtained EST-SSR markers can facilitate and promote related research such as ecological investigation, genetic diversity assessment and breeding practice of as well.

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