文献类型： 外文期刊

作者： Song, Yang ¹ ; Xie, Chun-hua ¹ ; Wang, Min-si ¹ ; Liu, Shuang ¹ ; Zhang, Zhi-jun ² ; Zhou, Yong-bin ³ ;

作者机构： 1.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China

2.Natl Engn & Technol Res Ctr Preservat Agr Prod, Tianjin Key Lab Postharvest Physiol & Storage Agr, Tianjin 300384, Peoples R China

3.Tianjin Forestry & Fruit Res Inst, Tianjin 300384, Peoples R China

期刊名称：2018 INTERNATIONAL SEMINAR ON FOOD SAFETY AND ENVIRONMENTAL ENGINEERING (FSEE 2018)

ISSN： 2267-1242

年卷期： 2019 年 78 卷

页码：

收录情况： SCI

摘要： In this study, the testing time was reduced to 1 h through optimizing, and an LOD of 0.3 +/- 0.15 mu g.L-1 and an IC50 of 2.7 +/- 0.3 mu g.L-1 were achieved by the optimized CD-ELISA. The sample extraction of 1-fold acetonitrile followed by a 20-fold dilution in assay diluted buffer has proven to be sufficient to eliminate the influence of matrix. The CD-ELISA was validated further by comparing with the standard HPLC. The average recovery ratio of 89.7%similar to 112.7% was obtained, and the coefficients of variation were less than 11.0%. In actual samples detection, except for Pleurotus eryngii, carbendazim was not detected, and other mushrooms were all detected, and only carbendazim in Volvariella volvacea exceeded the standard, and the over-standard rate was 16.7 %. In a word, the rapid, sensitive and efficient CD-ELISA for quantifing carbendazim in mushrooms was established in the study.