中国水产科学研究院机构知识库

Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification

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文献类型：外文期刊

作者： Zhang Fengying ¹ ; Shi Yanhong ¹ ; Jiang Keji ¹ ; Xu Zhaoli ¹ ; Ma Lingbo ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

2.Minist Agr China, Key & Open Lab Marine & Estuarine Fisheries, Shanghai 200090, Peoples R China

关键词： Alexandrium catenella;Alexandrium minutum;detection;loop-mediated isothermal amplification (LAMP);ribosomal DNA;internal transcribed spacer (ITS)

期刊名称：ACTA OCEANOLOGICA SINICA （影响因子：1.431；五年影响因子：1.445 ）

ISSN： 0253-505X

年卷期： 2012 年 31 卷 2 期

页码：

收录情况： SCI

摘要： A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (PSP). Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65 degrees C. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive; or negative results. The detection limits of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum.

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