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Cloning and expression analysis of two different LhcSR genes involved in stress adaptation in an Antarctic microalga, Chlamydomonas sp ICE-L

文献类型： 外文期刊

作者： Mou, Shanli 1 ; Zhang, Xiaowen 2 ; Ye, Naihao 2 ; Dong, Meitao 3 ; Liang, Chengwei 4 ; Liang, Qiang 1 ; Miao, Jinlai 1 ;

作者机构： 1.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

3.Qingdao Agr Univ, Qingdao 266109, Peoples R China

4.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China

关键词： Chlamydomonas sp ICE-L;LhcSR genes;Cloning;QRT-PCR;Expression analysis

期刊名称：EXTREMOPHILES （ 影响因子：2.395； 五年影响因子：2.668 ）

ISSN： 1431-0651

年卷期： 2012 年 16 卷 2 期

页码：

收录情况： SCI

摘要： Light-harvesting complexes (LHCs) play essential roles in light capture and photoprotection. Although the functional diversity of individual LHCs in many plants has been well described, knowledge regarding the extent of this family in the majority of green algal groups is still limited. In this study, two different LhcSR genes, LhcSR1 and LhcSR2 from Chlamydomonas sp. ICE-L, were cloned from the total cDNA and characterized in response to high light (HL), low light (LL), UV-B radiation and high salinity. The lower F (v)/F (m) as well as the associated induction of non-photochemical quenching (NPQ), observed under those conditions, indicated that Chlamydomonas sp. ICE-L was under stress. Under HL stress, the expression of LhcSR1 and LhcSR2 increased rapidly from 0.5 h HL and reached a maximum after 3 h. In LL, LhcSR2 expression was up-regulated during the first 0.5 h after which it decreased, while the expression of LhcSR1 decreased gradually from the beginning of the experiment. In addition, the transcript levels of LhcSR1 and LhcSR2 increased under UV-B radiation and high salinity. These results showed that both genes were inducible and up-regulated under stress conditions. A higher NPQ was accompanied by the up-regulated LhcSR genes, suggesting that LhcSR plays a role in thermal energy dissipation. Overall, the results presented here suggest that LhcSR1 and LhcSR2 play a primary role in photoprotection in Chlamydomonas sp. ICE-L under stress conditions and provide an important basis for investigation of the adaptation mechanism of LhcSR in Antarctic green algae.

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