文献类型： 外文期刊
作者： Hong Wang 1 ; Ze Lin-Zhao 1 ; Ding Jie-An 2 ; Jing Lin-Wang 1 ; Bin Tong-Yang 3 ; Yuan Huan-Kang 1 ; Dong Xing-Zhang 1 ; Hai Chao-Song 1 ; Xiao Feng-Shan 1 ; Ai Dong-Qian 1 ;
作者机构： 1.Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Jilin, Peoples R China
2.Jilin Acad Agr Sci, Inst Anim & Vet Med, Jilin 136100, Jilin, Peoples R China
3.Changchun Sci Tech Univ, Coll Life Sci, Changchun 130600, Jilin, Peoples R China
关键词： Aeromonas veronii; Lip gene; Virulence; Adhesion
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.848； 五年影响因子：3.957 ）
ISSN： 0882-4010
年卷期： 2022 年 167 卷
页码：
收录情况： SCI
摘要： Aeromonas veronii (A. veronii) is a pathogen that can infect aquatic organisms and mammals and has caused irrecoverable economic losses to the aquaculture industry. The results of an epidemiological investigation showed that the number of cases of A. veronii have increased gradually in recent years, and its drug resistance and virulence has shown an upward trend. In this study, we constructed an A. veronii mutant strain Delta lip, by homologous recombination and studied its function. The results showed that there was no significant difference in the biofilm formation ability between the Delta lip and the wild-type strain, but the toxicity of the Delta lip to EPC cells and its ability to adhere to EPC cells were significantly reduced. The LD50 value of the Delta lip to zebrafish was 7.40-fold higher than that of the wild-type strain. In addition, after 24 h and 72 h, the bacterial loads of the Delta lip in the organs of crucian carp were significantly lower than those in the wild-type strain. In conclusion, the mutant strain Delta lip led to a decrease in the adhesion and virulence of the wild-type strain, which lays a foundation to further understand lip gene function and the pathogenic mechanism of A. veronii.
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