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Unamplified system for sensitive and typing detection of ASFV by the cascade platform that CRISPR-Cas12a combined with graphene field-effect transistor

文献类型： 外文期刊

作者： Wang, Hua 1 ; Sun, Yang 2 ; Zhou, Yuan 1 ; Liu, Yujie 1 ; Chen, Shuo 3 ; Sun, Wenbo 4 ; Zhang, Zidong 5 ; Guo, Junqing 6 ; Yang, Cheng 3 ; Li, Zhengping 2 ; Chen, Lei 1 ;

作者机构： 1.Shandong Normal Univ, Dept Life Sci, Coll Life Sci, 1 Daxue Rd, Jinan 250014, Shandong, Peoples R China

2.Univ Sci & Technol Beijing, Dept Chem & Biol Engn, 30 Xueyuan Rd, Beijing 100083, Peoples R China

3.Shandong Normal Univ, Dept Phys & Elect, 1 Daxue Rd, Jinan 250014, Shandong, Peoples R China

4.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, 202 Gongye North Rd, Jinan 250100, Peoples R China

5.Shandong Univ, Key Lab Liquid Solid Struct Evolut & Proc Mat, 17923 Jing Shi Rd, Jinan 250061, Peoples R China

6.Henan Acad Agr Sci, Lab Anim Immunol, 116 Huayuan Rd, Zhengzhou 450099, Peoples R China

关键词： ASFV; Graphene; Field-effect transistor; CRISPR/Cas12a; Typing; Amplification free detection

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：12.6； 五年影响因子：10.8 ）

ISSN： 0956-5663

年卷期： 2023 年 240 卷

页码：

收录情况： SCI

摘要： At present, the 100% case fatality and the cross-infection of virus strains make the ASFV 's harm to society continue to expand. The absence of an effective commercial vaccine poses early detection remains the most effective means of curbing ASFV infection. Here, we report a cascaded detection platform based on the CRISPRCas12a system combined with graphene field-effect transistor sensors. The cascade platform could detect ASFV as low as 0.5 aM within 30 min and achieve typing of wild and vaccine strains of ASFV in a single detection system. The evaluation of 16 clinical samples proved that, compared with the gold standard Real-time PCR method, this platform has outstanding advantages in sensitivity, specificity and typing. Combining CRISPRCas12a's high specificity with the bipolar electric field effect of graphene field-effect transistor, the cascade platform is expected to achieve clinical application in the field of DNA disease detection, and provides a new direction for multi-strain disease typing.

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