Biological activity of recombinant bovine IFN-& alpha; and inhibitory effect on BVDV in vitro
文献类型： 外文期刊
作者： Yin, Hua 1 ; Li, Shaowei 1 ; Chai, Chunxia 1 ; Zhang, Fan 1 ; Ma, Yanhua 1 ; Wu, Youzhi 1 ; Fu, Cun 1 ; Diao, Yun 1 ; Zhou, Yanyan 1 ; Zhang, Jinlong 1 ; Niu, Rui 1 ; Wang, Wei 1 ;
作者机构： 1.Inner Mongolia Univ, Sch Life Sci, State Key Lab Reprod Regulat & Breeding Grassland, Hohhot 010000, Peoples R China
2.Inner Mongolia Acad Agr & Anim Husb Sci, Vet Res Inst, Hohhot, Peoples R China
3.Inner Mongolia Med Univ, Basic Med Sch, Hohhot 010000, Peoples R China
关键词： IFN-& alpha; BVDV; Molecular characteristic; Antiviral activity; Interferon-stimulated gene
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.8； 五年影响因子：4.0 ）
ISSN： 0882-4010
年卷期： 2023 年 181 卷
页码：
收录情况： SCI
摘要： Type I interferon has great broad-spectrum antiviral ability and immunomodulatory function, and its receptors are expressed in almost all types of cells. Bovine viral diarrhea virus (BVDV) is an important pathogen causing significant economic losses in cattle. In this study, a recombinant expression plasmid carrying bovine interferona(BoIFN-a)gene was constructed and transformed into E. coli BL21 (DE3) competent cells. SDS-PAGE and Westernblotting analysis showed that the recombinant BoIFN-a protein (rBoIFN-a) was successfully expressed. It is about 36KD and exists in the form of inclusion body. When denatured, purified and renatured rBoIFN-a protein stimulated MDBK cells, the expression of interferon stimulating genes (ISGs) such as ISG15, OAS1, IFIT1, Mx1 and IFITM1 were significantly up-regulated, and reached the peak at 12 h (P< 0.001). MDBK cells were infected with BVDV with moi of 0.1 and 1.0, respectively. The virus proliferation was observed after pretreatment with rBoIFN-a protein and post-infection treatment. The results showed that the denatured, purified and renatured BoIFN-a protein had good biological activity and could inhibit the replication of BVDV in MDBK cells in vitro, which provided a basis for BoIFN-a as an antiviral drug, immune enhancer and clinical application of BVDV.
- 相关文献
作者其他论文 更多>>
-
Genetic characterization of bovine coronavirus strain isolated in Inner Mongolia of China
作者：Zhang, Fan;Chai, Chunxia;Niu, Rui;Diao, Yun;Zhou, Yanyan;Zhang, Jinlong;Wu, Youzhi;Ma, Yanhua;Zan, Xiaohui;Wang, Wei;Zhang, Fan;Feng, Lin;Yao, Chunming
关键词：Bovine coronavirus; Genetic characterization; Isolated strain; China
-
A Reasonable Rotation Fallow Mode Enhances the Complexity of the Soil Bacterial Network and Enriches Nitrogen-Cycling-Related Taxa
作者：Shi, Gongfu;Fang, Jing;Wei, Shuli;Zhang, Fan;Wu, Jianhui;Zhao, Lili;Zhao, Xiaoqing;Lu, Zhanyuan;Wei, Shuli;Cheng, Yuchen;Su, Shaofeng;Zhang, Xiangqian;Wang, Jianguo;Zhao, Xiaoqing;Lu, Zhanyuan
关键词：rotation fallow mode; soil characteristics; microbial communities; functional taxa
-
A Multi-Omics Analysis Revealed the Diversity of the MYB Transcription Factor Family's Evolution and Drought Resistance Pathways
作者：Zhang, Fan;Ma, Jie;Liu, Ying;Fang, Jing;Wei, Shuli;Xie, Rui;Han, Pingan;Zhao, Xiaoqing;Lu, Zhanyuan;Zhang, Fan;Ma, Jie;Liu, Ying;Fang, Jing;Wei, Shuli;Xie, Rui;Han, Pingan;Zhao, Xiaoqing;Lu, Zhanyuan;Zhang, Fan;Ma, Jie;Liu, Ying;Fang, Jing;Wei, Shuli;Xie, Rui;Han, Pingan;Zhao, Xiaoqing;Lu, Zhanyuan;Zhang, Fan;Ma, Jie;Liu, Ying;Fang, Jing;Wei, Shuli;Xie, Rui;Han, Pingan;Zhao, Xiaoqing;Lu, Zhanyuan;Ma, Jie;Liu, Ying;Fang, Jing;Wei, Shuli;Zhao, Xiaoqing;Lu, Zhanyuan;Ma, Jie;Liu, Ying;Fang, Jing;Wei, Shuli;Zhao, Xiaoqing;Lu, Zhanyuan;Bo, Suling
关键词：MYB transcription factor; drought; transcriptome; evolution; bioinformatics
-
Improving the Yield and Quality of Tomato by Using Organic Fertilizer and Silicon Compared to Reducing Chemical Nitrogen Fertilization
作者：Zhang, Fan;Liang, Yi;Dai, Zemin;Zhao, Yanli;Shi, Yu;Hou, Leiping;Zhang, Yi;Liu, Yan;Gao, Jing;Ahammed, Golam Jalal
关键词：organic fertilizer; mineral N fertilizer; fruit quality; silicon fertilizer; tomato
-
Analyzing the Diversity of MYB Family Response Strategies to Drought Stress in Different Flax Varieties Based on Transcriptome Data
作者：Zhang, Fan;Liu, Ying;Ma, Jie;Zhao, Xiaoqing;Lu, Zhanyuan;Zhang, Fan;Liu, Ying;Ma, Jie;Su, Shaofeng;Chen, Liyu;Cheng, Yuchen;Buter, Siqin;Zhao, Xiaoqing;Yi, Liuxi;Lu, Zhanyuan;Zhang, Fan;Liu, Ying;Ma, Jie;Su, Shaofeng;Chen, Liyu;Cheng, Yuchen;Buter, Siqin;Zhao, Xiaoqing;Lu, Zhanyuan;Yi, Liuxi
关键词：expression profile; functional annotation; heat map; transcription factor; WGCNA
-
Characterization of a Highly Virulent Noncytopathic Bovine Viral Diarrhea Virus 2b Isolate Detected in Cattle in Inner Mongolia, China
作者：Chai, Chunxia;Zhang, Fan;Diao, Yun;Zhou, Yanyan;Li, Shaowei;Yin, Hua;Zhang, Jinlong;Niu, Rui;Wang, Wei;Zhang, Fan
关键词：
-
Identification and characterization of stress responsive homeodomain leucine zipper transcription factors in Medicago truncatula
作者：Li, Xiao;Hou, Yiyao;Zhang, Fan;Li, Mingna;Kang, Junmei;Yang, Qingchuan;Long, Ruicai;Yi, Fengyan
关键词：HD-ZIP I transcription factor; Medicago truncatula; Expression profile; Abiotic stresses
