Determination of Nitrofurans Residue in Fishery Products Using High Performance Liquid Chromatography
文献类型： 外文期刊
作者： Huang, Xuan-yun 1 ; Huang, Dong-mei 1 ; Shi, Yong-fu 1 ; Feng, Bing 1 ; Li, Bing; Yu, Hui-juan 1 ; Xu, Jie 1 ; Wang, Y 1 ;
作者机构： 1.Minist Agr PR China, CAFS Aquat Prod Qual Inspect & Test Ctr Shanghai, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
2.Minist Agr PR China, CAFS Aquat Prod Qual In
关键词： furaltadone;nitrofurazone;nitrofurantoin;furazolidone;high performance liquid chromatography;fishery products;food safety
期刊名称：ADVANCES IN CHEMISTRY RESEARCH II, PTS 1-3
ISSN： 1022-6680
年卷期： 2012 年 554-556 卷
页码：
收录情况： SCI
摘要： Nitrofurans residue in fishery products were dangerous for human health and still remain a big challenge in China. However, there are a few available methods to detect nitrofurans residue in fishery products. This study aimed at developing a high performance liquid chromatography (HPLC) method to detect four nitrofurans (furaltadone, nitrofurazone, nitrofurantoin and furazolidone) residue in fishery products sensitively. Firstly the nitrofurans were extracted from samples with ethyl acetate and then concentrated with rotary evaporator. After mobile phase dissolved and normal hexane defatted, the analytes were quantified with an external standard method by HPLC. The results showed that the linear range was 0.025 similar to 1.000 mu g/mL with correlation coefficients all more than 0.998 and the quantification limits of furaltadone, nitrofurazone, nitrofurantoin, furazolidone, were 11.0, 6.00, 13.0 and 7.00 mu g/kg respectively. The method was used to detect the nitrofurans in fishery products prepared with recovery rates from 72.2% to 107.4% and relative standard deviation (RSD) varied from 6.2% to 10.4%. Therefore, this method can be used for monitoring nitrofurans in commercial fishery products, improving the safety of commercial fishery products in China.
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