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Genetic Mapping and Transcriptomic Analysis Revealed the Molecular Mechanism Underlying Leaf-Rolling and a Candidate Protein Phosphatase Gene for the Rolled Leaf-Dominant (RL-D) Mutant in Rice

文献类型： 外文期刊

作者： Gong, Xiaomei 1 ; Wang, Fanhua 1 ; Chen, Huamei 1 ; Liu, Xiulian 1 ; Zhang, Shuchang 1 ; Zhao, Junliang 2 ; Yi, Jicai 1 ;

作者机构： 1.South China Agr Univ, Coll Life Sci, Guangzhou 510642, Peoples R China

2.Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou 510640, Peoples R China

3.Guangdong Acad Agr Sci, Guangdong Key Lab New Technol Rice Breeding, Guangzhou 510640, Peoples R China

关键词： Rice; Rolled leaf-dominant; Gene mapping; Transcriptomic analysis; Protein phosphatase

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN： 0735-9640

年卷期：

页码：

收录情况： SCI

摘要： In rice, moderate leaf rolling improves photosynthesis and crop yield. However, the molecular mechanisms underlying this important agronomic trait remain incompletely understood. Here, we investigated a dominant rolled leaf mutant (RL-D) developed from Nipponbare rice (WT). From the six-leaf stage, the leaves of the mutant rolled inward, and abnormal sclerenchyma tissues developed on the abaxial side of the leaf midribs. Additionally, leaf length, plant height, grain weight, and chlorophyll content were significantly greater in the mutant as compared to the WT. Genetic mapping analysis suggested that the leaf-rolling trait in the RL-D mutant was controlled by a single dominant gene, which was located in a 743-kb region on rice chromosome 3. Re-sequencing analysis showed that one gene in the mapped region encoding a protein phosphatase, Os03g0395100 (herein designated OsPP2C), had base mutations in the first exon. These mutations may have produced a truncated form of the OsPP2C protein in RL-D. Further transcriptomic analysis revealed that several biological processes, especially secondary cell wall formation and protein phosphorylation, were overrepresented among the differentially expressed genes (DEGs) between the mutant and the wild type. qRT-PCR verification also demonstrated that specific genes associated with leaf polarity and secondary cell wall formation were differentially expressed in the mutant. This study presents a novel dominant rolled-leaf germplasm that may help to improve rice leaf morphology in the future. The results also suggested that the RL-D phenotype might result from abnormal sclerenchyma tissue development, possibly regulated by OsPP2C via the dephosphorylation pathway. This may present a novel mechanism underlying leaf-rolling in rice.

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