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Establishment of a Multiplex PCR and an Investigation of Co-Infection Rate of WSSV and IHHNV in Penaeid vannamei in Northern of Jiangsu

文献类型： 外文期刊

作者： Chen, Yi-Bing 1 ; Zhou, Jun-Fang 3 ; Wan, Xi-He 4 ; Gao, Song 2 ;

作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 25009, Peoples R China

2.Yangzhou Univ, Coll Vet Med, Yangzhou 25009, Peoples R China

3.Jiangsu Inst Oceanog, Nantong 226007, Peoples R China

4.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

关键词： White spot syndrome virus;infectious hypodermal and hematopoietic necrosis virus;multiplex PCR;co-infection;templates;Chin

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 2 期

页码：

收录情况： SCI

摘要： Based on the conservative regions in genome of WSSV and IIIHNV, two specific primer sets, respectively for the two viruses were designed and constructed. A multiplex PCR for co-detection of the two viruses with a specificity of (IHHNV, TSV, YHV,Vibrio Parahaemolyticus, etc.) and sensitivity of 1x10(3)] template copy numbers was subsequent established after serially optimization. WSSV and IHHNV with different template concentrations could be detected simultaneously. By the multiplex PCR method, 265 diseased shrimp samples and 169 healthy shrimp samples collected from the farms in the Northern area of Jiangsu province was investigated for WSSV and IHHNV co-infection rate in pond-cultured P. vannamei. The results show that the infection rate of WSSV was quite high (45 similar to 54%) whereas that of IHHNV was very low (6.9 similar to 13.7%). The result also indicated that there were 7 similar to 10% of diseased shrimp and 0 similar to 6.7% of healthy shrimp were co-infected with the two viruses indicated that co-infection of the two viruses was more popular in diseased shrimp than the healthy.

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