(-)-5-O-(3-O-beta-D-Glucopyranosylcaffeoyl)-quinic acid from the fruits of Lycium barbarum L. var. auranticarpum K. F. Ching: Purification, identification and in vitro bioactivities
文献类型： 外文期刊
作者： Hu, Yuhang 1 ; Kan, Xuhui 1 ; Yang, Tingting 1 ; Fan, Xia 1 ; Zhou, Wangting 1 ; Chen, Guijie 1 ; Yan, Yamei 2 ; Lu, Lu 2 ; Mi, Jia 2 ; Zeng, Xiaoxiong 1 ; Cao, Youlong 2 ;
作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China
2.Ningxia Acad Agr & Forestry Sci, Inst Wolfberry Engn Technol, Yinchuan 750002, Ningxia, Peoples R China
3.Natl Wolfberry Engn Res Ctr, Yinchuan 750002, Ningxia, Peoples R China
关键词： Lycium barbarum L. var. auranticarpum K. F. Ching; (-)-5-O-(3-O-beta-D-Glucopyranosylcaffeoyl)quinic acid; Purification; Antioxidant; Anti-inflammatory
期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）
ISSN： 0308-8146
年卷期： 2022 年 389 卷
页码：
收录情况： SCI
摘要： Chlorogenic acids are important phenolics in the fruits of wolfberry, but little attention has been paid on their glucosylated forms. In the present study, a glucosylated form of chlorogenic acid was isolated from the fruits of Lycium barbarum L. var. auranticarpum K. F. Ching (also called yellow wolfberry) and identified to be (-)-5-O-(3-O-beta-D-glucopyranosylcaffeoyl)-quinic acid (5-CQA-3'beta G) by high resolution mass spectrometry and nuclear magnetic resonance spectrometry. The content of 5-CQA-3'beta G in the dried fruit was determined as 0.0293 +/- 0.0015% by HPLC. In addition, 5-CQA-3'beta G showed a good scavenging capacity for 2,2' -azino-bis-(3-ethylbenzothiazoline-6-sulphonate) free radicals but had a relatively low reducing power and scavenging capacity for 2,2-diphenyl-1-picrylhydrazyl free radical. Moreover, the secretion of nitric oxide, tumor necrosis factor-alpha and interleukin-6 as well as related mRNA expression were reduced in lipopolysaccharide-stimulated RAW264.7 macrophage cells treated with 5-CQA-3' beta G. This is the first report describing purification, identification and bioactivity of glucosylated CQA from yellow wolfberry.
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