中国水产科学研究院机构知识库

Isolation and characterization of polymorphic microsatellite loci from cDNA library of Scylla paramamosain

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文献类型：外文期刊

作者： Ma, Chunyan ¹ ; Ma, Hongyu ¹ ; Ma, Lingbo ¹ ; Jiang, Keji ¹ ; Zhang, Fengying ¹ ; Song, Wei ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Key Lab Marine & Estuarine Fisheries Resources &, Minist Agr, Shanghai 200090, Peoples R China

关键词： Scylla paramamosain;microsatellite markers;cDNA;polymorphism

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （影响因子：0.573；五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 54 期

页码：

收录情况： SCI

摘要： In this study, we reported 36 novel polymorphic microsatellite markers derived from a cDNA library of Scylla paramamosain. The polymorphisms of these markers were detected in a sample of 24 individuals. A total of 159 alleles were detected with an average of 4.4 alleles per locus. The number of alleles per locus ranged from two to eight. The observed and expected heterozygosity per locus ranged from 0.100 to 1.000 and from 0.097 to 0.819, with an average of 0.679 and 0.625, respectively. Five loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.0014) and no significant linkage disequilibrium between pairs of loci was found. These loci will provide useful information for the studies on genetic diversity and structure, construction of genetic linkage maps and the effectively management of S. paramamosain.

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