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Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis

文献类型: 外文期刊

作者: Liu Yunguo 1 ; Li Junfeng 3 ; Ye Naihao 4 ;

作者机构: 1.Shandong Entry Exit Inspect & Quarantine Bur, Inspect & Quarantine Technol Ctr, Qingdao 266002, Peoples R China

2.Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China

3.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China

4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词: half-smooth tongue sole;Cynoglossus semilaevis;AFLP marker;genetic characteristics

期刊名称:CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY ( 影响因子:1.068; 五年影响因子:0.983 )

ISSN: 0254-4059

年卷期: 2011 年 29 卷 3 期

页码:

收录情况: SCI

摘要: The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis, based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals from four wild populations, Laizhou (LZ), Weihai (WH), Qingdao (QD), Rizhao (RZ), and one hatchery population, Mingbo (MB), were screened using eight different AFLP primer combinations. A total of 384 loci were screened in the five studied populations. 48.4%, 51.3%, 50.7%, 49.3% and 45.8% of these loci were polymorphic among the individuals tested in the LZ, WH, QD, RZ and MB populations, respectively. The number of polymorphic loci detected by single primer combinations ranged from 17 to 35. The average heterozygosity of the LZ, WH, QD, RZ and MB populations was 0.072, 0.093, 0.092, 0.090 and 0.063, respectively. The WH population showed the highest genetic diversity in terms of total number of AFLP bands, total number of polymorphic bands, average heterozygosity and percentage of low frequency (0-0.2) polymorphic loci among all the populations, while the LZ population was the lowest among the wild populations. Compared with the wild populations, the hatchery population showed a low genetic viability.

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