Amide alkaloids characterization and neuroprotective properties of Piper nigrum L.: A comparative study with fruits, pericarp, stalks and leaves
文献类型： 外文期刊
作者： Yu, Lan 1 ; Hu, Xiaolu 1 ; Xu, Rongrong 2 ; Ba, Yinying 1 ; Chen, Xiaoqing 1 ; Wang, Xing 1 ; Cao, Bing 3 ; Wu, Xia 1 ;
作者机构： 1.Capital Med Univ, Sch Tradit Chinese Med, Beijing Key Lab TCM Collateral Dis Theory Res, 10 Youanmenwai, Beijing 100069, Peoples R China
2.Univ Sci & Technol China, Anhui Prov Canc Hosp, Affiliated Hosp USTC 1, Div Life Sci & Med,Dept Pharm, 107 Huanhu East Rd, Hefei 230031, Peoples R China
3.Hainan Univ, Hainan Acad Agr Sci, 14 Xingdan Rd, Haikou 571100, Hainan, Peoples R China
关键词： P; nigrum; Amide alkaloids; UHPLC-LTQ-Orbitrap HRMS; UHPSFC-MS; Neuroprotection
期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）
ISSN： 0308-8146
年卷期： 2022 年 368 卷
页码：
收录情况： SCI
摘要： Piper nigrum L. is commonly used worldwide and its pericarp, stalks, leaves will be major wastes materials. 42 amide alkaloids were identified in black, white pepper and pericarp by UHPLC-LTQ-Orbitrap HRMS method, followed by 40 constituents in stalks and 36 constituents in leaves. 8 amide alkaloids were reported for the first time in P. nigrum. An ultra-high-performance supercritical fluid chromatography (UHPSFC)-MS method was firstly applied to simultaneously determine 9 characteristic constituents (piperine, piperlonguminine, piperanine, pipercallosine, dehydropipernonaline, pipernonatine, retrofractamide B, pellitorine and guineensine). The most abundant compound in each extract was piperine with a concentration from 0.10 to 12.37 mg/g of dry weight. The fruits, pericarp and leaves extracts could improve cell viability in 6-OHDA-induced SK-N-SH and SH-SY5Y cells. The results showed the characteristics of amide alkaloids of different parts of P. nigrum and evaluated their neuroprotective activities.
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