OsPIP2-1 positively regulates rice seed germination by modulating OsAmy3C and α-amylase expression
文献类型： 外文期刊
作者： Chen, Bingxian 1 ; Peng, Yuanxuan 1 ; Zhang, Qi 1 ; Chen, Zhongjian 1 ; Li, Hongmei 2 ; Liu, Jun 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou, Guangdong, Peoples R China
2.Zhongkai Univ Agr & Engn, Coll Agr & Biol, Guangzhou, Peoples R China
关键词：
rice; seed germination; nanosilver;
期刊名称：PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：7.0 ）
ISSN： 0960-7412
年卷期： 2025 年 123 卷 1 期
页码：
收录情况： SCI
摘要： As timely germination of seeds is crucial to crop yield and quality, the low germination percentage for direct seeding in rice and preharvest sprouting during the late stage of maturity have extremely adverse effects on rice production. Herein, we found that an aquaporin gene OsPIP2-1 was significantly expressed during rice seed germination. The OsPIP2-1 overexpression seeds showed a higher germination rate, while the knockout lines showed a delayed germination phenotype, indicating that this gene positively regulates seed germination. Furthermore, in seeds of OsPIP2-1 overexpression and knockout lines, the activity of alpha-amylase and the expression of its encoding genes, OsAmy1C and OsAmy1E, were respectively enhanced and suppressed. When OsAmy3C was knocked out in the OsPIP2-1 overexpression lines, seed germination was significantly inhibited. Also, the germination of seeds with OsAmy3C knocked out in the wild-type background was delayed, and the expression level of OsPIP2-1 in these seeds remained almost unchanged during germination, suggesting that OsPIP2-1 is upstream of OsAmy1C. In addition, the ABA-biosynthesis genes OsNCED2/3 were reduced in OsPIP2-1 overexpression lines but raised in knockouts, while the ABA-catabolism gene OsABA8ox1-3 exhibited the opposite trend. Moreover, we have also applied nanosilver, a potential inhibitor of aquaporins, which effectively delays rice seed germination. At the molecular level, the expression of OsPIP2-1 in transcriptional and translational levels was significantly decreased by AgNPs. Meanwhile, the expression of alpha-amylase and its gene expression were also inhibited by AgNPs. In conclusion, OsPIP2-1 positively regulates rice seed germination by regulating OsAmy3C and alpha-amylase. It can enhance seed germination vigor in direct-seeded rice cultivation. Nanosilver delays germination by affecting OsPIP2-1 and alpha-amylase, thus offering a potential strategy for preventing preharvest sprouting.
- 相关文献
作者其他论文 更多>>
-
The Malectin-like kinase gene MdMDS1 negatively regulates the resistance of Pyrus betulifolia to Valsa canker by promoting the expression of PbePME1
作者：Zheng, Yan;Zhao, Dan;Lu, Yuan;Liu, Zhihong;Sun, E.;Yu, Hongqiang;Mao, Xia;Cai, Minrui;Zuo, Cunwu;Chen, Zhongjian;Zuo, Cunwu
关键词：
-
The Characterization of the Purine Nucleoside Phosphorylase from Agaricus bisporus and Its Potential Application in Reducing Purine Content in Beer
作者：Liu, Jun;Lu, Jian;Liu, Jun;Lu, Jian;Liu, Jun;Lu, Jian;Liu, Jun;Liu, Jun
关键词：beer; gout; purines; Agaricus bisporus; purine nucleoside phosphorylase
-
Integrated metabolomic and transcriptomic analysis of Pogostemon cablin shed new light on the complete biosynthesis pathway of pogostone
作者：Wang, Sen;Zeng, Zhaoqi;Zhang, Qi;Liu, Wenhua;Liu, Qinjian;Xie, Chong;Bei, Jinlong;Chen, Bingxian;Zhang, Aixia;Zeng, Zhaoqi;Xie, Chong
关键词：patchoulol; pogostone; biosynthesis; key enzyme; transcriptomics; patchouli; metabolomics
-
The Coexistence of Bicellular and Tricellular Pollen Might Be the Third Type of Pollen Cell Number: Evidence from Annonaceae
作者：Gan, Yangying;Zhang, Qi;Xiao, Chunfen;Ping, Jingyao
关键词：Annonaceae; pollen cell number; bicellular pollen; tricellular pollen
-
METTL3-dependent m6A modification of GHR mRNA regulates mitochondrial function through mitochondrial biogenesis during myoblast differentiation
作者：Zhao, Changbin;Hu, Bowen;Wang, Zhijun;Zhang, Ze;Luo, Wen;Li, Hongmei;Zhang, Xiquan;Zhao, Changbin;Hu, Bowen;Wang, Zhijun;Zhang, Ze;Luo, Wen;Li, Hongmei;Zhang, Xiquan;Zhao, Changbin;Hu, Bowen;Wang, Zhijun;Zhang, Ze;Luo, Wen;Li, Hongmei;Zhang, Xiquan
关键词：GHR; METTL3; m6A; Mitochondrial biogenesis; Mitochondrial function
-
Identification and characterization of a broadly neutralizing and protective nanobody against the HA1 domain of H5 avian influenza virus hemagglutinin
作者：Xu, Siqi;Liu, Yutong;Zhou, Mengruo;Wang, Ke;Xie, Qianmei;Zhang, Qi;Zhang, Qinying;Li, Qianyu;Pan, Zhichao;Feng, Saixiang;Liao, Ming;Luo, Chenying;Liao, Ming;Liao, Ming
关键词：influenza virus; yeast two-hybrid; nanobody; broadly neutralizing; surface plasmon resonance; inhibition mechanism; prophylactic; therapeutic; epitope mapping
-
Pretreatment with nano-silver extends the post-harvest longevity of gladiolus cut flowers by reducing free water mobility
作者：Li, Xiumei;Liu, Qinjian;Chen, Yuanyuan;Chen, Zhongjian;Liu, Jun;Yan, Shijuan;Li, Hongbo;He, Shenggen
关键词：Water state; Nanoparticles; Vase life; Nuclear magnetic resonance; Cut flowers
