Recombinant nanobody expressed by transgenic chimeric chickens eliminated vertical transmission of avian leukosis virus subgroup J
文献类型： 外文期刊
作者： Liang, Jiahua 1 ; Qin, Ke 1 ; Xiao, Ning 2 ; Zhao, Yingying 1 ; Han, Ruiying 1 ; Wang, Jinhan 1 ; Liu, Jiahan 1 ; Lu, Huicheng 1 ; Wang, Xiaoli 1 ; Yu, Fuxian 3 ; Tang, Xiaochuan 1 ;
作者机构： 1.Guangxi Univ, Coll Anim Sci & Technol, 100 East Daxue Rd, Nanning 530004, Guangxi, Peoples R China
2.Guangxi Acad Sci, Nanning, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou, Peoples R China
关键词： Avian leukosis virus subgroup J; Nanobody; Adenovirus; Transgenic chimeric chickens; Vertical transmission
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）
ISSN： 0378-1135
年卷期： 2025 年 302 卷
页码：
收录情况： SCI
摘要： Because of the vertical transmission of avian leukosis virus subgroup J (ALV-J), control of ALV-J in breed of chicken is still a serious issue. Blocking vertical transmission using antibodies is a potential strategy, but its high cost limits its application. We artificially designed recombinant nanobody (Nb) and efficiently expressed and secreted them in three primary chicken cells cultured in vitro by adenovirus delivery. We found that secreted the Nb could reduce the infection of cells by ALV-J. Moreover, we prepared transgenic chimeric chickens expressing Nb and found that the Nb reduced the infection and vertical transmission of ALV-J to chickens. In particular, transgenic chimeric hens expressing high concentration of the Nb eliminated vertical transmission of the virus. Collectively, current study provides new avenues for the control of ALV-J transmission.
- 相关文献
作者其他论文 更多>>
-
Statistical Analysis of Reproductive Traits in Jinwu Pig and Identification of Genome-Wide Association Loci
作者：Chen, Wenduo;Zhao, Ayong;Chen, Wenduo;Zhao, Ayong;Pan, Jianzhi;Tan, Kai;Zhu, Zhiwei;Zhang, Liang;Yu, Fuxian;Liu, Renhu;Huang, Jing;Zhong, Liepeng
关键词：reproductive traits; genome-wide association analysis; Jinwu pig; SNPs
-
The Influence of Increasing Roughage Content in the Diet on the Growth Performance and Intestinal Flora of Jinwu and Duroc x Landrace x Yorkshire Pigs
作者：Xu, Gaili;Chen, Wenduo;Zhao, Ayong;Xu, Gaili;Chen, Wenduo;Zhao, Ayong;Huang, Jing;Pan, Jianzhi;Yu, Fuxian
关键词：roughage diet; Jinwu pig; Duroc x Landrace x Yorkshire pig; roughage tolerance; gut microbiota
-
Xylanase Supplement Enhances the Growth Performance of Broiler by Modulating Serum Metabolism, Intestinal Health, Short-Chain Fatty Acid Composition, and Microbiota
作者：Wang, Xiaoli;Li, Danlei;Xu, Yibin;Ding, Xiaoqing;Liang, Shuang;Xie, Lingyu;Zhan, Xiuan;Wang, Yongxia
关键词：xylanase; growth performance; digestive absorption; intestinal barrier; metabolism
-
Comprehensive identification and risk assessment of regulated and emerging mycotoxins in infant foods and related raw materials and risk management advice: A case study of an infant food company in China
作者：Ji, Xiaofeng;Xiao, Yingping;Wei, Wang;Lyu, Wentao;Wang, Xiaoli;Yang, Hua;Yang, Weikang;Yang, Hua
关键词：Infant food; Wheat; Cereals; Mycotoxins; Food safety; Risk management
-
Emerging Alternaria and Fusarium mycotoxins in tomatoes and derived tomato products from the China market: Occurrence, methods of determination, and risk evaluation
作者：Ji, Xiaofeng;Xiao, Yingping;Lyu, Wentao;Wang, Wen;Wang, Xiaoli;Yang, Hua;Deng, Tao;Wu, Zhen;Jin, Canghong;He, Qinfang;Yang, Hua
关键词：Emerging; Alternaria; Fusarium; Mycotoxin; Tomato; Dietary exposure
-
Ileal Microbiota Alters the Immunity Statues to Affect Body Weight in Muscovy Ducks
作者：Fu, Zixian;Yang, Hua;Xiao, Yingping;Wang, Xiaoli;Wang, Wen;Lyu, Wentao;Fu, Zixian;Yang, Caimei;Lu, Lizhi
关键词：body weight; ileal microbiota; Muscovy ducks; microbial composition; growth-related bacteria; immunity
-
Mycotoxins in cereal-based infant foods marketed in China: Occurrence and risk assessment
作者：Ji, Xiaofeng;Xiao, Yingping;Wang, Wen;Lyu, Wentao;Wang, Xiaoli;Deng, Tao;Yang, Hua;Li, Yan;Li, Yan;Yang, Hua
关键词：Mycotoxin; Infant food; Dietary exposure; Food safety
