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Effects of luxS gene on growth characteristics, biofilm formation, and antimicrobial resistance of multi-antimicrobial-resistant Vibrio parahaemolyticus Vp2015094 isolated from shellfish

文献类型： 外文期刊

作者： Jiang, Yanhua 1 ; Wang, Peng 2 ; Qu, Meng 1 ; Wang, Ting 1 ; Li, Fengling 1 ; Wang, Lianzhu 1 ; Yao, Lin 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, Minist Agr & Rural Affairs, Qingdao 266071, Peoples R China

2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

关键词： Vibrio parahaemolyticus; luxS; biofilm; antimicrobial resistance; conjugation; transcriptome sequencing

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：4.0； 五年影响因子：4.3 ）

ISSN： 1364-5072

年卷期： 2023 年 134 卷 8 期

页码：

收录情况： SCI

摘要： Aims: Vibrio parahaemolyticus is an important foodborne pathogen worldwide, which can cause gastroenteritis. This study aimed to investigate the effect of quorum sensing system LuxS/AI-2-related gene luxS on the biological characteristics and antimicrobial resistance of V. parahaemolyticus Vp2015094 from shellfish, which carried a multi-antimicrobial-resistant plasmid. Methods and Results: The critical gene luxS related to the synthesis of AI-2 in V. parahaemolyticus Vp2015094 was knocked out by homologous recombination with suicide plasmid. The effect of luxS on the biological characteristics of V. parahaemolyticus was determined by comparing the growth, AI-2 activity, motility, biofilm formation ability, and antibiotic resistance between the wildtype strain and the luxS deletion mutant. Compared with wildtype strain, the production of AI-2, the motility and biofilm formation ability, antimicrobial resistance, and conjugation frequency of luxS deletion mutant strain were decreased. The transcriptome sequencing showed that the transcriptional levels of many genes related to motility, biofilm formation, antimicrobial resistance, and conjugation were significantly downregulated after luxS deletion. Conclusions: Quorum sensing system LuxS/AI-2-related gene luxS in V. parahaemolyticus Vp2015094 played an important role in growth characteristics, biofilm formation, antimicrobial resistance, and resistance genes' transfer. Significance and Impact of Study The critical gene luxS related to quorum sensing system LuxS/AI-2 could regulate the biological characteristics of bacteria including the antimicrobial resistance and resistance genes' transfer, which provided a new pathway to study the pathogenicity and antimicrobial resistance mechanisms and develop the related control techniques of Vibrio parahaemolyticus

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