Upgrading the genome of an elite japonica rice variety Kongyu 131 for lodging resistance improvement
文献类型： 外文期刊
作者： Wang, Chen 1 ; Feng, Xiaomin 1 ; Yuan, Qingbo 1 ; Lin, Kangxue 1 ; Zhang, Xiaohui 1 ; Yan, Li 1 ; Nan, Jianzong 1 ; Zhang, Wenqi 1 ; Wang, Rongsheng 1 ; Wang, Lihong 1 ; Xue, Qian 1 ; Yang, Xiaowen 1 ; Liu, Zhixia 2 ; Lin, Shaoyang 1 ;
作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing, Peoples R China
2.Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou, Peoples R China
3.Guangdong Key Lab New Technol Rice Breeding, Guangzhou, Peoples R China
关键词： rice; lodging resistance; fine mapping; CRISPR/Cas9; upgrade
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.263； 五年影响因子：11.619 ）
ISSN： 1467-7644
年卷期：
页码：
收录情况： SCI
摘要： Developing a new rice variety requires tremendous efforts and years of input. To improve the defect traits of the excellent varieties becomes more cost and time efficient than breeding a completely new variety. Kongyu 131 is a high-performing japonica variety with early maturity, high yield, wide adaptability and cold resistance, but the poor-lodging resistance hinders the industrial production of Kongyu 131 in the Northeastern China. In this study, we attempted to improve the lodging resistance of Kongyu 131 from perspectives of both gene and trait. On the one hand, by QTL analysis and fine mapping we discovered the candidate gene loci. The following CRISPR/Cas9 and transgenic complementation study confirmed that Sd1 dominated the lodging resistance and favourable allele was mined for precise introduction and improvement. On the other hand, the Sd1 allelic variant was identified in Kongyu 131 by sequence alignment, then introduced another excellent allelic variation by backcrossing. Then, the two new resulting Kongyu 131 went through the field evaluation under different environments, planting densities and nitrogen fertilizer conditions. The results showed that the plant height of upgraded Kongyu 131 was 17%-26% lower than Kongyu 131 without penalty in yield. This study demonstrated a precise and targeted way to update the rice genome and upgrade the elite rice varieties by improving only a few gene defects from the perspective of breeding.
- 相关文献
作者其他论文 更多>>
-
The highly allo-autopolyploid modern sugarcane genome and very recent allopolyploidization in Saccharum
作者：Zhang, Jisen;Hua, Xiuting;Wang, Baiyu;Yu, Zehuai;Gao, Ruiting;Wang, Tianyou;Zhang, Qing;Li, Zhen;Li, Yihan;Xu, Yi;Yao, Wei;Zhang, Muqing;Chen, Baoshan;Qi, Yiying;Wang, Yongjun;Wang, Yuhao;Li, Shuangyu;Qi, Nameng;Feng, Xiaoxi;Wu, Mingxing;Du, Cuicui;Deng, Zuhu;Qi, Yongwen;Huang, Yumin;Zhang, Yixing;Mei, Jing;Pan, Haoran;Liu, Jia;Chen, Shuqi;Fang, Yaxue;Ma, Panpan;Sun, Yuanchang;Ming, Ray;Tang, Haibao;Wang, Gang;Li, Qingyun;Feng, Xiaomin;Liu, Xinlong;Wang, Jianping
关键词：
-
Advances in the Structure, Function, and Regulatory Mechanism of Plant Plasma Membrane Intrinsic Proteins
作者：Li, Xueting;Guo, Yirong;Ling, Qiuping;Lei, Yawen;Wu, Jiayun;Zhang, Nannan;Ling, Qiuping;Zhang, Nannan;Guo, Zhejun;Feng, Xiaomin
关键词：plasma membrane intrinsic proteins; structure and function; molecular mechanism; regulatory mechanism
-
Full-length transcriptome integrated with RNA-seq reveals potassium deficiency stress-regulated key pathways and time-specific responsive genes in sugarcane roots
作者：Feng, Xiaomin;Zeng, Qiaoying;Chen, Xinglong;Wu, Zilin;Zhang, Nannan;Huang, Yonghong;Wu, Jiayun;Feng, Xiaomin;Shan, Miaomiao;Shan, Miaomiao;Feng, Xiaomin;Chen, Xinglong;Wu, Zilin;Zhang, Nannan
关键词：Sugarcane; Low potassium stress; Transcriptome; Signal transduction; CIPK23
-
Hehongzhan 2: a red rice variety for South China with blast and brown planthopper resistance
作者：Hu, Wei;Gao, Yun;Liu, Zhixia;Zhang, Qiang;Luo, Kebo
关键词：Red rice; breeding; brown planthopper-resistance gene; blast resistance; cultivation methods
-
A new strategy of molecular breeding for optimal heading date and grain yield in rice by modulating elite allelic combinations of Ghd7, Hd3a, RFT1 and Gn1a
作者：Su, Qingmei;Zhang, Yuhang;Zhu, Li;Qian, Qian;Su, Qingmei;Zhao, Mengyue;Zhu, Guanlin;Zhang, Fang;Chen, Fan;Wang, Rongsheng;Qian, Qian;Chen, Fan;Feng, Xiaomin;Wang, Qing;Qian, Qian;Chen, Fan;Wang, Rongsheng;Feng, Xiaomin;Lin, Shaoyang;Zhang, Yuhang;Zhu, Li;Qian, Qian
关键词：Adaptation; Elite gene combinations; Oryza sativa; Photoperiod response
-
QTL mapping and candidate gene analysis of element accumulation in rice grains via genome-wide association study and population genetic analysis
作者：Li, Min;Gao, Mengxue;Huang, Jinmei;Jin, Shaojuan;Lv, Yamei;Wang, Lin;Huang, Tao;Wu, Fengcai;Zhang, Chaopu;Meng, Lijun;Xu, Jianlong;Xu, Jianlong;Xu, Zhijian;Pan, Yinghua;Liang, Yuntao;Liu, Zhixia
关键词：Element accumulation; GWAS; QTL; Divergent region; Gene
-
Development of a TaqMan polymerase chain reaction detection method for the precise identification and quantification of an attenuated Eimeria maxima vaccine strain in poultry
作者：Jin, Haozhan;Cai, Haiming;Liao, Shenquan;Qi, Nanshan;Li, Juan;Lv, Minna;Lin, Xuhui;Hu, Junjing;Song, Yongle;Zhu, Yibin;Chen, Xiangjie;Yin, Lijun;Zhang, Xiaohui;Zhang, Jianfei;Sun, Mingfei;Jin, Haozhan;Zhang, Xu
关键词：avian coccidiosis; Eimeria maxima; attenuated live oocyst vaccines; molecular marker; TaqMan PCR
