Molecular cloning, genomic structure and expression analysis of major histocompatibility complex class I alpha gene of half-smooth tongue sole (Cynoglossus semilaevis)
文献类型: 外文期刊
作者: Xu, Tian-jun 2 ; Chen, Song-lin 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China
2.Zhejiang Ocean Univ, Coll Marine Sci, Key Lab Marine Living Resources & Mol Engn, Zhoushan 316000, Peoples R China
关键词: Half-smooth tongue sole;MHC I alpha;Cloning;Sequence analysis;Gene expression profile
期刊名称:FISH PHYSIOLOGY AND BIOCHEMISTRY ( 影响因子:2.794; 五年影响因子:2.876 )
ISSN: 0920-1742
年卷期: 2011 年 37 卷 1 期
页码:
收录情况: SCI
摘要: Major histocompatibility complex (MHC) has a central role in the adaptive immune system by presenting foreign peptide to the T-cell receptor. The full length of MHC class I alpha cDNA was cloned from half-smooth tongue sole by homology cloning and rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR), genomic organization and expression of MHC I alpha were examined to study the function of MHC gene in fish. The domain structure feature and antigen-binding motifs of other teleost and mammals MHC are conserved in the half-smooth tongue sole MHC I alpha gene. The deduced amino acid sequence of half-smooth tongue sole MHC I alpha (GenBank accession no. FJ372720) had 12.1-61.8% identity with those of human and other fish. Eight exons and seven introns were identified in MHC I alpha gene. Real-time quantitative PCR demonstrated that MHC I alpha gene was ubiquitously expressed in normal tissues, while that in Vibrio anguillarum infected fish was significantly increased in intestines and decreased in spleen and liver from 24 to 72 h after infection, followed by a recovery to normal level after 96 h.
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