文献类型： 外文期刊

作者： Liu, Zerui ¹ ; Guo, Yu ¹ ; Qin, Chuanxin ¹ ; Mu, Xiaohui ¹ ; Zhang, Jia ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China

2.Sanya Trop Fisheries Res Inst, Key Lab Efficient Utilizat & Proc Marine Fishery R, Sanya 572018, Peoples R China

3.Shanghai Ocean Univ, Coll Marine Sci, Shanghai 201306, Peoples R China

4.Hainan Yazhou Bay Seed Lab, Sanya 572025, Peoples R China

关键词： purple sea urchin; isotope; 16S rDNA; 18S rDNA

期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）

ISSN：

年卷期： 2024 年 13 卷 8 期

页码：

收录情况： SCI

摘要： Preliminary investigations revealed that purple sea urchins are distributed in both the stone and algal areas of Daya Bay, with a greater density in the stone area than in the large algal area. This raises important scientific questions: What do the purple sea urchins in the stone area feed on? What is the dietary range of purple sea urchins? Therefore, we focused on purple sea urchins and employed stable isotope technology and 16S rDNA and 18S rDNA high-throughput sequencing techniques to conduct a systematic study on the feeding habits and gut microbiota community structure of purple sea urchins in the stone and algal areas of the central islet sea area of Daya Bay. We aimed to elucidate the feeding habits and dietary range of purple sea urchins. The results of this study provide a theoretical basis for the restoration of wild purple sea urchin resources and the selection of areas for restocking and release. Sea urchins play an important role in marine ecosystems. Owing to limitations in previous research methods, there has been insufficient understanding of the food sources and ecological functional value of purple sea urchins, leading to considerable controversy regarding their functional positioning. We focused on Daya Bay as the research area, utilizing stable isotope technology and high-throughput sequencing of 16S rDNA and 18S rDNA to analyze sea urchins and their potential food sources in stone and algae areas. The results showed that the delta C-13 range of purple sea urchins in the stone area is -11.42 similar to-8.17 parts per thousand, and the delta N-15 range is 9.15 similar to 10.31 parts per thousand. However, in the algal area, the delta C-13 range is -13.97 similar to-12.44 parts per thousand, and the delta N-15 range is 8.75 similar to 10.14 parts per thousand. There was a significant difference in delta C-13 between the two areas (p < 0.05), but there was no significant difference in delta N-15 (p > 0.05). The main food source for purple sea urchins in both areas is sediment. The sequencing results of 18S rDNA revealed that, in the algal area, the highest proportion in the sea urchin gut was Molluska (57.37%). In the stone area, the highest proportion was Arthropoda (76.71%). The sequencing results of 16S rDNA revealed that, in the algal area, Bacteroidetes was the dominant group in the sea urchin gut (28.87%), whereas, in the stone area, Proteobacteria was the dominant group (37.83%). Diversity detection revealed a significant difference in the number of gut microbes and eukaryotes between the stone and algal areas (p < 0.05). The results revealed that the main food source of purple sea urchins in both areas is sediment, but the organic nutritional value is greater in the algal area, and the richness of microbiota and eukaryotes in the gut of purple sea urchins in the stone area is greater. These results indicated that purple sea urchins are likely omnivores and that the area where they occur impacts their growth and development. The results of this study provide a theoretical basis for the restoration of wild purple sea urchin resources and the selection of areas for restocking and release.