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Fine mapping and analysis of a candidate gene controlling Phytophthora blight resistance in cucumber

文献类型： 外文期刊

作者： Wang, R. 1 ; Lin, Y. 'e. 2 ; Zhang, C. 1 ; Wu, H. 1 ; Jin, Q. 2 ; Guo, J. 1 ; Cao, H. 1 ; Tan, D. 1 ; Wu, T. 1 ;

作者机构： 1.Guangdong Acad Agr Sci IFA GDAAS, Inst Facil Agr, Guangzhou, Peoples R China

2.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou, Peoples R China

3.Guangdong Acad Agr Sci IFA GDAAS, Inst Facil Agr, Guangzhou 510640, Peoples R China

关键词： Cucumber blight; fine mapping; Phytophthora melonis; resistance gene

期刊名称：PLANT BIOLOGY （ 影响因子：3.9； 五年影响因子：3.6 ）

ISSN： 1435-8603

年卷期： 2024 年 26 卷 4 期

页码：

收录情况： SCI

摘要： center dot Cucumber blight is a destructive disease. The best way to control this disease is resistance breeding. This study focuses on disease resistance gene mapping and molecular marker development. center dot We used the resistant cucumber, JSH, and susceptible cucumber, B80, as parents to construct F2 populations. Bulked segregant analysis (BSA) combined with specific length amplified fragment sequencing (SLAF-seq) were used, from which we developed cleaved amplified polymorphic sequence (CAPs) markers to map the resistance gene. center dot Resistance in F2 individuals showed a segregation ratio of resistance:susceptibility close to 3:1. The gene in JSH resistant cucumber was mapped to an interval of 9.25 kb, and sequencing results for the three genes in the mapped region revealed three mutations at base sites 225, 302, and 591 in the coding region of Csa5G139130 between JSH and B80, but no mutations in coding regions of Csa5G139140 and Csa5G139150. The mutations caused changes in amino acids 75 and 101 of the protein encoded by Csa5G139130, suggesting that Csa5G139130 is the most likely resistance candidate gene. We developed a molecular marker, CAPs-4, as a closely linked marker for the cucumber blight resistance gene. center dot This is the first report on mapping of a cucumber blight resistance gene and will provideg a useful marker for molecular breeding of cucumber resistance to Phytophthora blight.

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