您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Pathogenicity and detection method based on interferon release assay of Mycobacteria marinum isolated from sturgeon

文献类型： 外文期刊

作者： Xie, Meng 1 ; Deng, Dan 1 ; Li, Qian 1 ; Zhang, Xiao 1 ; Luo, Kai 1 ; Zhou, Jiang 1 ; Li, Chenghua 2 ; Wang, Bei 3 ; Wei, Qiwei 4 ; Xu, Qiaoqing 1 ; Zhang, Shuhuan 5 ;

作者机构： 1.Yangtze Univ, Inst Chinese Sturgeon Dis, Jingzhou 434024, Peoples R China

2.Ningbo Univ, Natl Engn Res Lab Marine biotechnol & Engn, Ningbo 315211, Peoples R China

3.Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China

4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat Minist Agr, Minist Agr, Wuhan 430223, Peoples R China

5.Guizhou Univ Tradit Chinese Med, Sturgeon Hlth Breeding & Med Value Res Ctr, Guiyang 550025, Peoples R China

关键词： Chinese sturgeon; Mycobacterium marinum; Antimicrobial-response; Disease detection

期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）

ISSN： 0044-8486

年卷期： 2023 年 577 卷

页码：

收录情况： SCI

摘要： Mycobacterium marinum is an opportunistic pathogen and can infect marine and freshwater fish. In this study, an acid-fast bacillus strain was isolated from the moribund Acipenser sinensis and identified as M. marinum via specific plate culture and phylogenetic analysis of 16S rRNA. M. marinum exhibited pathogenicity in Chinese sturgeon and hybrid sturgeon (A. schrenckii & FEMALE; x A. baerii & MALE;). The histopathology results showed that granulo-matous nodules were observed in the liver, spleen and kidney of the Chinese sturgeon after M. marinum infection. Nuclear necrosis and large production of hemosiderin appeared in the liver. The medullary structure of the spleen was disordered. The glomerulus was deformed and disintegrated, and the glomerular lumen was dilated in the kidney. The expressions of IFN-e1, IFN-& gamma;, TNF & alpha;1, TNF & alpha;2, IL-1 & beta;, and IL-6 of Chinese sturgeon were signifi-cantly upregulated by M. marinum in vivo and in vitro. The whole blood ELISA detection method was constructed by IFN-& gamma; polyclonal antibody. The infection of M. marinum in hybrid sturgeon could be judged by detecting the concentration of IFN-& gamma; in plasma. In summary, M. marinum has a strong pathogenicity to sturgeon and could induce the immune response of the host. The sturgeon IFN-& gamma;-ELISA detection method was expected to become a tool for diagnosing M. marinum infection in sturgeon. This study could provide the scientific basis for effectively controlling and reducing the pathogenic M. marinum disease of sturgeon in aquaculture.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台