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Electrochemical characterization of a truncated hydrogenase from Pyrococcus furiosus

文献类型： 外文期刊

作者： Wang, Yuanming 1 ; Song, Yunhong 2 ; Ma, Chunling 2 ; Xia, Hong-qi 3 ; Wu, Ranran 2 ; Zhu, Zhiguang 1 ;

作者机构： 1.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China

2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, 32 West 7th Ave, Tianjin 300308, Peoples R China

3.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou 510640, Peoples R China

关键词： [NiFe] hydrogenase; Truncated hydrogenase; Enzymatic fuel cell; Hydrogen oxidation; Electron transfer rate

期刊名称：ELECTROCHIMICA ACTA （ 影响因子：6.901； 五年影响因子：6.016 ）

ISSN： 0013-4686

年卷期： 2021 年 387 卷

页码：

收录情况： SCI

摘要： A highly thermostable cytoplasmic [NiFe] hydrogenase PfSHI, incorporating four subunits (alpha beta gamma delta), displays a clear direct electron transfer (DET) type bioelectrocatalytic activity. In order to investigate the biological and electrochemical properties of the corresponding truncated hydrogenases, we constructed two truncated enzymes (i.e. Pf(alpha delta), and Pf(alpha)) derived from the native hydrogenase and immobilized them on multiwalled carbon nanotubes casted glassy carbon electrodes. The cyclic voltammogram along with the enzymatic activity assay revealed that the dimer Pf(alpha delta) showed an obvious oxidation current under H-2 in a wide temperature range, demonstrating its DET capability, while the monomer Pf(alpha), had no activity in H-2 oxidation. At high potentials, Pf(alpha delta) was proved to be inactive and observed no reverse recovery on the return scan under 40 to 60 degrees C. Notably, Pf(alpha delta) still maintained oxygen tolerance to some extent and its activity can be recovered after applying a reduction potential. Further, enzyme turnover frequencies and kinetic parameters were analyzed based on a steady state model, which revealed that Pf(alpha delta) exhibited a higher electron transfer rate than that of pristine PfSHI due to the shortened electron transfer distance between the enzyme and the electrode. (C) 2021 Elsevier Ltd. All rights reserved.

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