文献类型： 外文期刊

作者： Ma, Lingyan ¹ ; Lyu, Wentao ¹ ; Zeng, Tao ² ; Wang, Wen ¹ ; Chen, Qu ¹ ; Zhao, Jiangchao ³ ; Zhang, Guolong ⁴ ; Lu, Lizhi ² ; Yang, Hua ¹ ; Xiao, Yingping ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Hangzhou 310021, Peoples R China

3.Univ Arkansas, Dept Anim Sci, Div Agr, Fayetteville, AR USA

4.Oklahoma State Univ, Dept Anim & Food Sci, Stillwater, OK USA

关键词： duck; gastrointestinal microbiome; intestinal regional; metagenome-assembled genomes; rearing condition; temporal development

期刊名称：IMETA （ 影响因子：33.2； 五年影响因子：33.2 ）

ISSN： 2770-5986

年卷期： 2024 年 3 卷 4 期

页码：

收录情况： SCI

摘要： The duck gastrointestinal tract (GIT) harbors an abundance of microorganisms that play an important role in duck health and production. Here, we constructed the first relatively comprehensive duck gut microbial gene catalog (24 million genes) and 4437 metagenome-assembled genomes using 375 GIT metagenomic samples from four different duck breeds across five intestinal segments under two distinct rearing conditions. We further characterized the intestinal region-specific microbial taxonomy and their assigned functions, as well as the temporal development and maturation of the duck gut microbiome. Our metagenomic analysis revealed the similarity within the microbiota of the foregut and hindgut compartments, but distinctive taxonomic and functional differences between distinct intestinal segments. In addition, we found a significant shift in the microbiota composition of newly hatched ducks (3 days), followed by increased diversity and enhanced stability across growth stages (14, 42, and 70 days), indicating that the intestinal microbiota develops into a relatively mature and stable community as the host duck matures. Comparing the impact of different rearing conditions (with and without water) on duck cecal microbiota communities and functions, we found that the bacterial capacity for lipopolysaccharide biosynthesis was significantly increased in ducks that had free access to water, leading to the accumulation of pathogenic bacteria and antibiotic-resistance genes. Taken together, our findings expand the understanding of the microbiome signatures linked to intestinal regional, temporal development, and rearing conditions in ducks, which highlight the significant impact of microbiota on poultry health and production. In this study, we constructed the first relatively comprehensive duck gut microbial gene catalog and metagenome-assembled genomes using 375 duck gastrointestinal tract metagenomic samples from four different duck breeds across five intestinal segments under two distinct rearing conditions. Furthermore, the present study expands the understanding of the duck microbiome signatures linked to intestinal regional, temporal development, and rearing conditions in ducks, highlighting the significant impact of microbiota on poultry health and production. image A catalog of microbial genes and metagenome-assembled genomes from the duck gut microbiome. The microbiota within different duck intestinal segments exhibits both similarities and distinctive taxonomic and functional differences. Significant shifts in microbiota composition have been observed in newly hatched ducks at 3 days of age, followed by increased diversity and enhanced stability across growth stages (14, 42, and 70 days). Ducks with free access to water have shown an increased bacterial capacity for lipopolysaccharide biosynthesis, leading to the accumulation of pathogenic bacteria and antibiotic-resistance genes.