中国水产科学研究院机构知识库

Isolation and characterization of 45 polymorphic microsatellite loci of turbot (Scophthalmus maximus) and cross-species amplification

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文献类型：外文期刊

作者： Hou Shiying ¹ ; Ma Aijun ¹ ; Wang Xin'an ¹ ; Huang Zhihui ¹ ; Xue Baogui ¹ ; Yang Zhi ¹ ; Qu Jiangbo ² ;

作者机构： 1.Chinese Acad Fishery Sci, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Yantian Tianyuan Aquat Ltd Corp, Yantai 264003, Peoples R China

3.Rushan Fisheries Tech Extens Stn, Rushan 264500, Peoples R China

关键词： turbot;Scophthalmus maximus;enriched library;microsatellite

期刊名称：CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY （影响因子：1.068；五年影响因子：0.983 ）

ISSN： 0254-4059

年卷期： 2011 年 29 卷 2 期

页码：

收录情况： SCI

摘要： Turbot (Scophthalmus maximus) is a flatfish species commercially important for aquaculture. In this study, we generated a microsatellite-enriched genomic DNA library for Scophthalmus maximus, and then isolated and characterized 45 microsatellite loci by genotyping 30 individuals. The observed number of alleles ranged from 2 to 19 with an average of 6.24, while the effective number of alleles ranged from 1.30 to 11.11 with an average of 3.66. The expected heterozygosities varied from 0.23 5 to 0.925 4 and polymorphic information content ranged from 0.204 4 to 0.903 3, with an average of 0.622. Twelve loci deviated significantly from Hardy-Weinberg equilibrium, and no significant linkage disequilibrium was observed between any pair of loci after Bonferroni correction. In cross-species amplification, five flatfish species (Paralichthys lethostigma, Verasper moseri, platichthys stellatus, Hippoglossoides dub ius and Cynoglossus semilaevis) showed at least one polymorphic locus. These polymorphic microsatellite loci should prove useful for population analysis of turbot and other related species.

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