cDNA Cloning and Expression Analysis of Gustavus Gene in the Oriental River Prawn Macrobrachium nipponense
文献类型： 外文期刊
作者： Zhang, Fengying 1 ; Chen, Liqiao 1 ; Qin, Jianguang 3 ; Zhao, Weihong 1 ; Wu, Ping 1 ; Yu, Na 1 ; Ma, Lingbo 2 ;
作者机构： 1.E China Normal Univ, Coll Life Sci, Shanghai 200062, Peoples R China
2.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Shanghai, Peoples R China
3.Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2011 年 6 卷 2 期
页码：
收录情况： SCI
摘要： The gustavus gene is required for localizing pole plasm and specifying germ cells. Research on gustavus gene expression will advance our understanding of the biological function of gustavus in animals. A cDNA encoding gustavus protein was identified and termed MnGus in the oriental river prawn Macrobrachium nipponense. Bioinformatic analyses showed that this gene encoded a protein of 262 amino acids and the protein belongs to the Spsb1 family. Real-time quantitative PCR analyses revealed that the expression level of MnGus in prawn embryos was slightly higher at the cleavage stage than at the blastula stage, and reached the maximum level during the zoea stage of embryos. The minimum level of MnGus expression occurred during the perinucleolus stage in the ovary, while the maximum was at the oil globule stage, and then the level of MnGus expression gradually decreased with the advancement of ovarian development. The expression level of MnGus in muscle was much higher than that in other tissues in mature prawn. The gustavus cDNA sequence was firstly cloned from the oriental river prawn and the pattern of gene expression was described during oocyte maturation, embryonic development, and in other tissues. The differential expression patterns of MnGus in the embryo, ovary and other somatic tissues suggest that the gustavus gene performs multiple physiological functions in the oriental river prawn.
- 相关文献
作者其他论文 更多>>
-
Muti-Biomarker Approach and IBR Index to Evaluate the Oxidative Stress of Nanoplastic on the Clams, Meretrix meretrix and Sinonovacula constricta
作者：Lv, Linlan;Feng, Wanjun;Sun, Ke;Zhao, Weihong;Jiang, Fengjuan;Yang, Yue;Zhang, Yingying;Sui, Yanming;Dong, Xuexing;Mohamed, Mohsen;Mohamed, Mohsen;Li, Lei
关键词：biomarker; antioxidant enzyme;
Meretrix meretrix ;Sinonovacula constricta ; nanoplastics; oxidative stress -
COMPARATIVE ANALYSIS OF THE GROWTH PERFORMANCE IN THE PORTUNIDAE) UNDER DIFFERENT SEX RATIOS PROVIDES NEW INSIGHTS FOR CRAB AQUACULTURE: A LABORATORY STUDY
作者：Luo, Minghao;Jin, Xin;Ma, Lingbo;Luo, Minghao;Wang, Xueyang;Liu, Zhiqiang;Jin, Xin;Wang, Wei;Ma, Lingbo;Zhao, Ming
关键词：Scylla paramamosain; aquaculture; sex ratio; growth performance; survival ratio
-
Integration of metagenome and full-length 16S rRNA gene sequencing of the mariculture environmental bacterial community reveal potential pathogenic bacteria in broodstock breeding of the mud crab, Scylla paramamosain
作者：Xu, Likun;Hu, Xin;Ma, Chunyan;Wang, Wei;Zhang, Fengying;Zhao, Ming;Ma, Lingbo;Ma, Keyi;Liu, Zhiqiang;Xu, Jiayuan;Lv, Xiaokang;Su, Zhixing
关键词：Scylla paramamosain; Bacteria; Metagenomic sequencing; Full-length 16S rRNA; Pathogenic bacteria
-
Comparative analysis of phytoplankton community structure and stability in the Arabian Sea and Bay of Bengal using eDNA metabarcoding
作者：Li, Changwen;Xiao, Ge;Ai, Tongxi;Tang, Fenghua;Ju, Jianfeng;Yang, Shenglong;Gao, Lei;Ma, Lingbo;Fan, Wei;Zhao, Ming;Zheng, Hanfeng;Jiang, Keji;Li, Changwen;Ai, Tongxi;Ju, Jianfeng;Long, Han;Pi, Yan
关键词：eDNA; AS; BB; Phytoplankton; Diversity; Stability
-
THE EFFECT OF DIFFERENT SALINITIES ON THE GILL MORPHOLOGY AND RELATED GENE EXPRESSION OF LARVAL SCYLLA PARAMAMOSAIN ESTAMPADOR, 1950 (BRACHYURA, PORTUNIDAE)
作者：Yin, Chunyan;Shi, Fangfang;Liu, Zhiqiang;Zhao, Ming;Ma, Chunyan;Ma, Lingbo;Wang, Wei;Zhang, Fengying;Yin, Chunyan;Shi, Fangfang
关键词：Salinity; gene expression; Na plus -K plus -Cl--cotransporter (NKCC); carbonic anhydrase (CA)
-
Effects of estradiol on fatty acid composition and tissue structure of hepatopancreas in Procambarus clarkii
作者：Zhao, Weihong;Liu, Jintao;Zheng, Xirui;Lv, Linlan;Chen, Jiyi;Wang, Jiamei;Sui, Yanming;Zhao, Weihong;Zheng, Liang
关键词：Procambarus clarkii; Estrogen; Fatty acid; Hepatopancreas; Histology
-
Feeding rhythm of the zoea larvae of Scylla paramamosain: The dynamic feeding rhythm is not completely synchronized with photoperiod
作者：Wang, Wei;Liu, Zhiqiang;Wang, Xueyang;Zhang, Fengying;Ma, Chunyan;Zhao, Ming;Ma, Keyi;Ma, Lingbo
关键词：Scylla paramamosain; Zoea; Feeding rhythm; Feeding rate; Digestive enzyme
