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Set1/COMPASS regulates growth, pathogenicity, and patulin biosynthesis of Penicillium expansum via H3K4 methylation and the interaction with PeVelB

文献类型： 外文期刊

作者： Xu, Xiaodi 1 ; Chen, Yong 1 ; Zhang, Zhanquan 1 ; Chen, Tong 1 ; Li, Boqiang 1 ; Tian, Shiping 1 ;

作者机构： 1.Chinese Acad Sci, Inst Bot, State Key Lab Plant Divers & Specialty Crops, Beijing 100093, Peoples R China

2.China Natl Bot Garden, Beijing 100093, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Inst Agrifood Proc & Nutr, Beijing Vegetable Res Ctr,Beijing Key Lab Fruits &, Key Lab Vegetable Postharvest Proc,Minist Agr & Ru, Beijing 100097, Peoples R China

关键词： Penicillium expansum; Set1/COMPASS; Interaction; Regulation

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：11.4； 五年影响因子：11.0 ）

ISSN： 2090-1232

年卷期： 2024 年 62 卷

页码：

收录情况： SCI

摘要： Introduction: Penicillium expansum is a harmful plant fungal pathogen that causes blue mold disease and produces mycotoxin patulin, leading to huge economic losses and food safety hazard. Set1 associated complex Set1/COMPASS deposits the methylation at lysine 4 of histone H3, which is associated with gene expression in diverse biological processes of fungi. However, the function and underlying mechanisms of Set1/COMPASS are poorly defined in P. expansum. Objectives: The study aimed to identify Set1/COMPASS and investigate its regulation mechanisms on growth, pathogenicity, and patulin biosynthesis of P. expansum. Methods: Analyses of phylogenetic relationship, conserved structural domain, and gene deletion were used to identify components of Set1/COMPASS. Phenotype analysis and stress tolerance test of gene deletion mutants were conducted to analyze the function of these components. Yeast two-hybrid, CoImmunoprecipitation (Co-IP), and point mutation were performed to verify the protein interaction. Western blot was conducted for detection of H3K4 methylation levels. Results: P. expansum owns six components of Set1/COMPASS besides PeSet1. Absence of each component resulted in reduction of H3K4 methylation levels and impaired growth, pathogenicity, and patulin biosynthesis, as well as altered stress responses of P. expansum. One component PeBre2p was found to interact with the conserved global regulator PeVelB (VelvetLike protein B) at Asp294 of PeBre2p. This interaction affected fungal growth and utilization of fructose, lactose, glycine, and proline in P. expansum. Conclusion: This study revealed the important roles of Set1/COMPASS in P. expansum and clarified for the first time the combined regulation of PeBre2p and PeVelB in fungal growth and nutrition utilization. These results will provide potential targets for the control of blue mold disease. (c) 2024 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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