文献类型： 外文期刊

作者： Gong, Ruiguang ¹ ; Ye, Xiangyang ² ; Wang, Shuhui ¹ ; Ren, Zhanjun ¹ ;

作者机构： 1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China

2.Guangdong Acad Agr Sci, State Key Lab Livestock & Poultry Breeding, Inst Anim Sci,Key Lab Anim Nutr & Feed Sci South, Minist Agr & Rural Affairs,Guangdong Key Lab Anim, Guanghzou, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.752； 五年影响因子：4.069 ）

ISSN： 1932-6203

年卷期： 2021 年 16 卷 11 期

页码：

收录情况： SCI

摘要： In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiological, biochemical, and stress resistance were studied. The results showed that the bacterium was Clostridium sartagoforme, a gram positive anaerobe, which can produce spores. The colony diameter was 0.5 mm-2.5 mm, the diameter of the bacteria was 0.5 mu m-1.0 mu m x 2.0 mu m-6.3 mu m, and the spore diameter was 1 mu m-1.2 mu m x 1 mu m-1.2 mu m. C. sartagoforme can utilize various sugars and alcohols such as fructose, galactose, sorbitol, and inositol. It secreted cellulase into the extracellular environment to form a transparent hydrolysis circle in Congo red medium, it could not liquify gelatin, and the lysine decarboxylase reaction was positive. In liquid medium it entered the stable growth period after 9 h of inoculation. Additionally, it had good stress resistance with a survival rate that exceeded 53% after gastric juice (pH 2.5) treatment for 3 h, it grew in a medium with a bile salt concentration of 0.3%, and the survival rate exceeded 85% after 10 minutes at 80 degrees C. Moreover, animal testing indicated that this strain has no adverse effects on the morbidity and mortality of rabbits. In summary, C. sartagoforme XN-T4 was isolated from rabbit feces. This bacterium has good resistance to stress, can decompose a variety of monosaccharides and polysaccharides including cellulose, which is relatively harmless for animal health.