文献类型： 外文期刊

作者： Guo, Lifei ¹ ; Lu, Liang ¹ ; Wang, Huichao ¹ ; Zhang, Xiaoxing ¹ ; Wang, Genan ¹ ; Zhao, Tingbin ⁵ ; Zheng, Guobao ⁴ ; Qiao, Changsheng ¹ ;

作者机构： 1.Tianjin Univ Sci & Technol, Coll Bioengn, Tianjin 300457, Peoples R China

2.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Engn Res Ctr Microbial Metab & Fermentat P, Tianjin 300457, Peoples R China

3.Tianjin Univ Sci & Technol, State Key Lab Biobased Fiber Mfg Technol, Tianjin 300457, Peoples R China

4.Ningxia Acad Agr & Forestry Sci, Inst Forestry Sci, Agr Biotechnol Res Ctr, Yinchuan 750002, Peoples R China

5.Tianjin Huizhi Biotrans Bioengn Co Ltd, Tianjin 300457, Peoples R China

关键词： Poly-gamma-glutamic acid (gamma-PGA); Bacillus licheniformis; Fermentation; Sugarcane molasses; FeSO4; 7H(2)O; Metabolomics; Transcriptome

期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：6.4； 五年影响因子：6.3 ）

ISSN：

年卷期： 2023 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background Poly-gamma-glutamic acid (gamma-PGA) is biodegradable, water-soluble, environment-friendly, and edible. Consequently, it has a variety of industrial applications. It is crucial to control production cost and increase output for industrial production gamma-PGA.Results Here gamma-PGA production from sugarcane molasses by Bacillus licheniformis CGMCC NO. 23967 was studied in shake-flasks and bioreactors, the results indicate that the yield of gamma-PGA could reach 40.668 g/L in a 5L stirred tank fermenter. Further study found that gamma-PGA production reached 70.436 g/L, gamma-PGA production and cell growth increased by 73.20% and 55.44%, respectively, after FeSO4 & BULL;7H(2)O was added. Therefore, we investigated the metabolomic and transcriptomic changes following FeSO4 & BULL;7H(2)O addition. This addition resulted in increased abundance of intracellular metabolites, including amino acids, organic acids, and key TCA cycle intermediates, as well as upregulation of the glycolysis pathway and TCA cycle.Conclusions These results compare favorably with those obtained from glucose and other forms of biomass feedstock, confirming that sugarcane molasses can be used as an economical substrate without any pretreatment. The addition of FeSO4 & BULL;7H(2)O to sugarcane molasses may increase the efficiency of gamma-PGA production in intracellular.