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cDNA cloning and mRNA expression of a tandem-repeat galectin (PoGal2) from the pearl oyster, Pinctada fucata

文献类型： 外文期刊

作者： Zhang, D. C. 1 ; Hu, Y. T. 1 ; Guo, H. Y. 1 ; Cui, S. G. 1 ; Su, T. F. 1 ; Jiang, S. G. 1 ;

作者机构： 1.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Aquaculture & Biotechnol Div, Guangzhou, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China

3.Jinan Univ, Sch Life Sci & Technol, Guangzhou, Guangdong, Peoples R China

关键词： Pinctada fucata;Pearl oyster;Lectin;beta-galactoside;Innate immunity

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2011 年 10 卷 3 期

页码：

收录情况： SCI

摘要： Galectins can recognize and specifically bind to beta-galactoside residues, playing crucial roles in innate immune responses of vertebrates and invertebrates. We cloned the cDNA of a tandem-repeat galectin from the pearl oyster Pinctada fucata (designated as PoGal2). PoGal2 cDNA is 1347 bp long and consists of a 5'-untranslated region (UTR) of 3 bp, a 3'-UTR of 297 bp with one cytokine RNA instability motif (ATTTA), and an open reading frame of 1047 bp, encoding a polypeptide of 349 amino acids, with an estimated molecular mass of 38.1 kDa and a theoretical isoelectric point of 8.5. PoGal2 contains two carbohydrate recognition domains (CRDs); both have the conserved carbohydrate-binding motifs H-NPR and WG-EE. PoGal2 shares 50.6 and 50.9% identity with those of abalone (Haliotis discus) and the Manila clam (Venerupis philippinarum), respectively. Phylogenetic analysis revealed that the tandem-repeat galectins formed two clades for the different species. Molluscan tandem-repeat galectins were clustered into a single clade, and nematode tandem-repeat galectins were clustered into another single clade. In both clades, CRD-N and CRD-C were divided into different groups. PoGal2 mRNA was constitutively expressed in all tissues analyzed, and the expression level of PoGal2 mRNA was found to be significantly up-regulated in digestive glands, gills and hemocytes after Vibrio alginolyticus stimulation/infection. Expression profile analysis showed that the expression level of PoGal2 mRNA was significantly up-regulated at 8, 12 and 24 h after V. alginolyticus infection. These results suggest that PoGal2 is a constitutive and inducible acute-phase protein involved in the innate immune response of pearl oysters.

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[8]EXPRESSION, PURIFICATION, AND FUNCTIONAL ACTIVITY OF SHELL MATRIX PROTEIN PEARLIN FROM THE PEARL OYSTER PINCTADA FUCATA. Huang, Guiju,Bi, Xiaomin,Zhang, Bo,Qu, Tao,Liu, Baosuo,Fan, Sigang,Yu, Dahui,Bi, Xiaomin.

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