您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A Dirigent Gene, ZmDIR11, Positively Regulates Drought Tolerance in Maize

文献类型： 外文期刊

作者： Zhao, Zhixiong 1 ; Guan, Yuan 1 ; Qin, Tao 1 ; Zheng, Hongjian 1 ; Wang, Hui 1 ; Xu, Wen 3 ; Gu, Wei 1 ; Yu, Diansi 1 ; Wei, Jihui 1 ; Hu, Yinxiong 1 ;

作者机构： 1.Cultivat Res Inst, Crop Breeding, Shanghai 201403, Peoples R China

2.Shanghai Acad Agr Sci, CIMMYT China Specialty Maize Res Ctr, Shanghai Engn Res Ctr Specialty Maize, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201403, Peoples R China

3.Xinjiang Agr Univ, Coll Agr, Urumqi 830052, Peoples R China

关键词： dirigent protein; DIR gene; ZmDIR11; drought tolerance

期刊名称：AGRONOMY-BASEL （ 影响因子：3.4； 五年影响因子：3.8 ）

ISSN：

年卷期： 2025 年 15 卷 3 期

页码：

收录情况： SCI

摘要： The DIR gene family, which encodes Dirigent proteins, plays a crucial role in plant development and stress responses. However, the functions and mechanisms of this family in maize remained underexplored. This study identified ZmDIR11, a member of the maize Dirigent protein family, and explored its role in drought tolerance. ZmDIR11 shared conserved regions with homologous proteins in wheat, rice, and Arabidopsis. RT-qPCR analysis revealed that ZmDIR11 expression is upregulated in leaves under drought and PEG stress, while subcellular localization confirmed its presence in the endoplasmic reticulum. Virus-induced gene silencing (VIGS) and EMS mutagenesis demonstrated that ZmDIR11 silencing or mutation significantly reduces drought tolerance in maize seedlings, indicating its positive regulatory role. Silencing or mutation of ZmDIR11 led to decreased growth parameters (plant height, root length, fresh weight, dry weight, and chlorophyll content) under drought stress, alongside a reduced antioxidant capacity, as evidenced by increased levels of MDA and ROS and decreased activities of SOD, CAT, and POD. Furthermore, ZmDIR11 mutation impaired the synthesis of ABA and zeatin, downregulating key genes in the ABA (ZmABA1, ZmNCED3, ZmSnRK2.6) and zeatin (ZmIPT4, ZmCKO5, ZmCKO4b) pathways. Drought-responsive genes (ZmRD20, ZmRD22, ZmDREB2A) and lignan biosynthesis genes (Zm4CL, ZmC3H, ZmCAD1) are also downregulated in ZmDIR11 mutants. In conclusion, ZmDIR11 enhances drought tolerance in maize by regulating antioxidant capacity, ABA and zeatin synthesis, and lignan metabolism. This study provides new insights into the role of DIR genes in drought tolerance and offers a potential genetic resource for breeding drought-resistant maize varieties.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台